Department of Botany and Microbiology, College of Science, King Saud University, 2460, Riyadh 11451, Saudi Arabia.
Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, 2460, Riyadh 11451, Saudi Arabia.
Biomolecules. 2021 Jan 30;11(2):197. doi: 10.3390/biom11020197.
Green synthesis of metal nanoparticles using plant extracts as capping and reducing agents for the biomedical applications has received considerable attention. Moreover, emergence and spread of multidrug resistance among bacterial pathogens has become a major health concern and lookout for novel alternative effective drugs has gained momentum. In current study, we synthesized gold nanoparticles using the seed extract of (TA-AuNPs), assessed its efficacy against drug resistant biofilms of and , and evaluated its anticancer potential against HepG2 cancer cell lines. Microwave-assisted green synthesis of gold nanoparticles was carried out and characterization was done using UV-vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and dynamic light scattering (DLS). Most nanoparticles were observed as spherical and spheroidal with few anisotropies with an average crystalline size of 16.63 nm. Synthesized TA-AuNPs demonstrated significant biofilm inhibitory activity against (73%) as well as (81%). Exopolysaccharide (EPS), motility, and CSH, key elements that facilitate the formation and maintenance of biofilm were also inhibited significantly at the tested sub-minimum inhibitory concentrations (sub-MICs). Further, TA-AuNPs effectively obliterated preformed mature biofilms of and by 64% and 58%, respectively. Induction of intracellular ROS production in TA-AuNPs treated bacterial cells could be the plausible mechanism for the reduced biofilm formation in test pathogens. Administration of TA-AuNPs resulted in the arrest of cellular proliferation in a concentration-dependent manner. TA-AuNPs decrease the intracellular GSH in HepG2 cancer cell lines, cells become more prone to ROS generation, hence induce apoptosis. Thus, this work proposes a new eco-friendly and rapid approach for fabricating NPs which can be exploited for multifarious biomedical applications.
利用植物提取物作为包覆和还原剂来合成金属纳米粒子的绿色方法，因其在生物医学应用方面的潜力而受到广泛关注。此外，细菌病原体中多药耐药性的出现和传播已成为一个主要的健康关注点，因此寻找新型有效的替代药物已成为当务之急。在本研究中，我们使用 （TA-AuNPs）的种子提取物合成了金纳米粒子，评估了其对耐药生物膜的疗效，并评估了其对 HepG2 癌细胞系的抗癌潜力。采用微波辅助绿色合成法合成金纳米粒子，并通过紫外可见分光光度法、X 射线衍射（XRD）、透射电子显微镜（TEM）和动态光散射（DLS）进行了表征。大多数纳米粒子呈球形和类球形，具有少数各向异性，平均结晶尺寸为 16.63nm。合成的 TA-AuNPs 对 （73%）和 （81%）的生物膜抑制活性显著。外多糖（EPS）、运动性和 CSH 等促进生物膜形成和维持的关键元素也在测试的亚最小抑菌浓度（sub-MICs）下显著受到抑制。此外，TA-AuNPs 有效地消除了 和 预先形成的成熟生物膜，分别为 64%和 58%。在经 TA-AuNPs 处理的细菌细胞中诱导细胞内 ROS 产生可能是测试病原体中生物膜形成减少的合理机制。TA-AuNPs 的处理导致细胞增殖以浓度依赖的方式受到抑制。TA-AuNPs 降低 HepG2 癌细胞系中的细胞内 GSH，细胞更容易产生 ROS，从而诱导细胞凋亡。因此，这项工作提出了一种新的环保和快速的方法来制造 NPs，可用于多种生物医学应用。
