Shin Tae-Gi, Park Jin-Sook
Department of Biological Sciences and Biotechnology, Hannam University Jeonmin-Dong, Yuseong-gu, Daejeon, 34430, Republic of Korea.
Curr Microbiol. 2021 Mar;78(3):1061-1067. doi: 10.1007/s00284-020-02328-1. Epub 2021 Feb 11.
A Gram-stain-negative, rod-shaped, non-motile, aerobic, yellow-pigmented, marine bacterium (designated strain 176CP5-101) was isolated from a sponge Hymeniacidon sinapium from Cheongpo beach, Taean in Republic of Korea. The strain was able to grow at pH 6.8-8.5 (optimum at pH 7.5), in 1-7% (w/v) NaCl (optimum at 3%, w/v) and at 10-30 °C (optimum at 30 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 176CP5-101 belonged to the family Flavobacteriaceae and was most closely related to Flagellimonas aquimarina KCTC 52351 (97.1%), F. algicola KACC 19790 (97.0%), F. pacifica KCTC 52762 (96.9%), F. eckloniae KCCM 42307 (96.4%), F. flava KCTC 22665 (96.0%), and F. maritima KCTC 32464 (95.4%). Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain 176CP5-101 and F. aquimarina KCTC 52351 were, respectively, 79.9% and 22.1%, indicating that 176CP5-101 is a novel species of the genus Flagellimonas. The DNA G + C content of the genomic DNA was 37.8 mol%. Menaquinone with six isoprene units (MK-6) was the major respiratory quinone, and the major polar lipids were phosphatidylethanolamine (PE), unidentified aminophospholipid, and unidentified lipids. The fatty acids are mainly (> 5%) defined iso-C, iso-C 3-OH, iso-C G, iso-C 3-OH, and C ω7c and/or C ω6c (summed feature 3). Phenotypic, phylogenetic, genomic, and chemotaxonomic characteristics showed that strain 176CP5-101 represents a novel species of the genus Flagellimonas, for which the name Flagellimonas hymeniacidonis sp. nov. is proposed. The type strain is 176CP5-101 (= KACC 19892 = LMG 31316).
从韩国泰安清浦海滩的海绵 Hymeniacidon sinapium 中分离出一株革兰氏阴性、杆状、无运动性、需氧、产黄色色素的海洋细菌（命名为菌株 176CP5 - 101）。该菌株能够在 pH 6.8 - 8.5（最适 pH 7.5）、1 - 7%（w/v）NaCl（最适 3%，w/v）以及 10 - 30 °C（最适 30 °C）条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 176CP5 - 101 属于黄杆菌科，与海生鞭毛单胞菌 KCTC 52351（97.1%）、藻栖鞭毛单胞菌 KACC 19790（97.0%）、太平洋鞭毛单胞菌 KCTC 52762（96.9%）、海带鞭毛单胞菌 KCCM 42307（96.4%）、微黄鞭毛单胞菌 KCTC 22665（96.0%）以及海生鞭毛单胞菌 KCTC 32464（95.4%）关系最为密切。菌株 176CP5 - 101 与海生鞭毛单胞菌 KCTC 52351 之间的平均核苷酸同一性（ANI）和数字 DNA - DNA 杂交（dDDH）值分别为 79.9%和 22.1%，表明 176CP5 - 101 是鞭毛单胞菌属的一个新物种。基因组 DNA 的 G + C 含量为 37.8 mol%。含有六个异戊二烯单元的甲萘醌（MK - 6）是主要的呼吸醌，主要极性脂质为磷脂酰乙醇胺（PE）、未鉴定的氨基磷脂和未鉴定的脂质。脂肪酸主要（>5%）为特征性异-C、异-C₃-OH、异-C₉、异-C₉-OH 以及 Cω₇c 和/或 Cω₆c（总和特征 3）。表型、系统发育、基因组和化学分类学特征表明，菌株 176CP5 - 101 代表鞭毛单胞菌属的一个新物种，为此提出新名称海栖海绵鞭毛单胞菌（Flagellimonas hymeniacidonis sp. nov.）。模式菌株为 176CP5 - 101（= KACC 19892 = LMG 31316）。
