Shin Ji-Yoon, Park Jin-Sook
Department of Biological Sciences and Biotechnology, Hannam University, Jeonmin-dong, Yuseong-gu, Daejeon 34430, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Feb;73(2). doi: 10.1099/ijsem.0.005702.
A novel Gram-stain-negative, thin-rod-shaped, aerobic, non-motile and yellow-pigmented marine bacterium (designated strain 2012CJ35-5) was isolated from a marine sponge sampled in Jeju-do, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 2012CJ35-5 belonged to the family , and was most closely related to KCCM 90075 (96.3 %), JCM 33425 (95.7 %), KCTC 22665 (95.6 %), KCTC 52351 (95.6 %) and KCTC 22266 (94.9 %). Average nucleotide identity, amino acid identities and digital DNA-DNA hybridization between strain 2012CJ35-5 and the closest related strain were 72.6, 73.6 and 17.3 % respectively, indicating that strain 2012CJ35-5 represents a novel species of the genus . The genome size of strain 2012CJ35-5 is 3.8 Mbp and the G+C content is 43.9 mol%. Strain 2012CJ35-5 contained menaquinone 6 as the major respiratory quinone and phosphatidylethanolamine, four unidentified aminophospholipids and five unidentified lipids as major polar lipids. The fatty acids were mainly (>15 %) defined as C iso (31.7 %), C iso G (29.2 %) and C iso 3OH (18.0 %). Strain 2012CJ35-5 could be distinguished from the other members of the genus by a number of chemotaxonomic and phenotypic characteristics. Based on the results of polyphasic taxonomic analysis, strain 2012CJ35-5 (=KACC 22643=LMG 32584) represents a novel species within the genus , for which the name sp. nov. is proposed.
从韩国济州岛采集的一块海洋海绵中分离出了一种新型革兰氏阴性、细杆状、需氧、不运动且产黄色色素的海洋细菌（命名为菌株2012CJ35-5）。基于16S rRNA基因序列的系统发育分析表明，菌株2012CJ35-5属于 科，与KCCM 90075（96.3%）、JCM 33425（95.7%）、KCTC 22665（95.6%）、KCTC 52351（95.6%）和KCTC 22266（94.9%）亲缘关系最近。菌株2012CJ35-5与最相近相关菌株之间的平均核苷酸同一性、氨基酸同一性和数字DNA-DNA杂交率分别为72.6%、73.6%和17.3%，这表明菌株2012CJ35-5代表了该属的一个新物种。菌株2012CJ35-5的基因组大小为3.8 Mbp，G+C含量为43.9 mol%。菌株2012CJ35-5含有甲基萘醌6作为主要呼吸醌，磷脂酰乙醇胺、四种未鉴定的氨基磷脂和五种未鉴定的脂质作为主要极性脂质。脂肪酸主要（>15%）为C异（31.7%）、C异G（29.2%）和C异3OH（18.0%）。菌株2012CJ35-5可通过多种化学分类和表型特征与该属的其他成员区分开来。基于多相分类分析结果，菌株2012CJ
