Institute of Histology and Embryology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
Department for diabetes and metabolic diseases, Clinic for Internal Medicine, University Medical Centre Maribor, Maribor, Slovenia.
Bosn J Basic Med Sci. 2021 Oct 1;21(5):642-646. doi: 10.17305/bjbms.2020.5368.
The global prevalence for diabetes mellitus nearly doubled from 4.7% in 1980 to 8.5% in 2014. Sirtuin 1 (SIRT1) is an NAD+-dependent deacetylase that is expressed in a variety of tissues. It modifies proteins that participate in DNA repair, stress, and inflammatory response. The aim of the study was to investigate the relationship between SIRT1 rs7069102 polymorphism and diabetic nephropathy (DN) in patients with type 2 diabetes mellitus (T2DM). In our retrospective association study, we included 724 Slovene (Caucasian) patients who have had T2DM for at least 10 years. We classified the participants into two groups, the first group was comprised of 301 patients with DN, and the second (control) group was comprised of 423 patients without DN. We analyzed the rs7069102 polymorphism using StepOne real-time polymerase chain reaction (PCR) System and TaqMan SNP Genotyping Assay. We found a statistically significant difference in the distribution of rs7069102 genotypes and alleles between the two groups. We used logistic regression analysis and adjusted for systolic pressure, arterial hypertension (AH), duration of AH, triglycerides, the value of HbA1c, carotid disease, diabetic foot, and diabetic retinopathy. Furthermore, we discovered that patients with the CC genotype are significantly more likely to develop DN according to both the codominant (odds ratio [OR] = 1.94; 95% confidence interval [CI] = 1.09-3.45; p = 0.02) and recessive (OR = 2.39; 95% CI = 1.12-5.08; p = 0.02) models of inheritance. We found a significant association between the SIRT1 rs7069102 polymorphism and DN in T2DM. We speculate that SIRT1 rs7069102 might be an interesting marker of DN.
全球糖尿病患病率从 1980 年的 4.7%几乎翻了一番，达到 2014 年的 8.5%。Sirtuin 1（SIRT1）是一种 NAD+依赖性脱乙酰酶，在多种组织中表达。它修饰参与 DNA 修复、应激和炎症反应的蛋白质。本研究旨在探讨 2 型糖尿病（T2DM）患者 SIRT1 rs7069102 多态性与糖尿病肾病（DN）之间的关系。在我们的回顾性关联研究中，我们纳入了 724 名至少患有 10 年 T2DM 的斯洛文尼亚（白种人）患者。我们将参与者分为两组，第一组由 301 名患有 DN 的患者组成，第二组（对照组）由 423 名无 DN 的患者组成。我们使用 StepOne 实时聚合酶链反应（PCR）系统和 TaqMan SNP 基因分型检测分析 rs7069102 多态性。我们发现两组之间 rs7069102 基因型和等位基因的分布存在统计学显著差异。我们使用逻辑回归分析，并调整了收缩压、动脉高血压（AH）、AH 持续时间、甘油三酯、HbA1c 值、颈动脉疾病、糖尿病足和糖尿病视网膜病变。此外，我们发现根据共显性（比值比[OR] = 1.94；95%置信区间[CI] = 1.09-3.45；p = 0.02）和隐性（OR = 2.39；95% CI = 1.12-5.08；p = 0.02）遗传模型，CC 基因型的患者发生 DN 的可能性明显更高。我们发现 SIRT1 rs7069102 多态性与 T2DM 中的 DN 之间存在显著关联。我们推测 SIRT1 rs7069102 可能是 DN 的一个有趣标志物。
