Chao P, Ammann D, Oesch U, Simon W, Lang F
Laboratorium für Organische Chemie, Eidgenössische Technische Hochschule Zürich (ETH), Switzerland.
Pflugers Arch. 1988 Feb;411(2):216-9. doi: 10.1007/BF00582318.
A series of new neutral hydrogen ion carriers suitable for application in H+-selective microelectrodes is presented. One carrier (ETH 1907) proves to be superior to tridodecylamine currently very much in use. Microelectrodes based on ETH 1907 in an optimized membrane composition exhibit a linear dynamic response function from pH 2 to 9 extended into the acidic range, a response time less than or equal to 5 s, and a resistance of about 35 G omega for a tip diameter of about 1 micron. This makes the electrode suitable for measurements at normal physiological intracellular pH as well as in acid physiological media. Measurements using this microelectrode in proximal tubule cells of isolated perfused frog kidney are presented.
本文介绍了一系列适用于氢离子选择性微电极的新型中性氢离子载体。其中一种载体（ETH 1907）被证明优于目前广泛使用的三（十二烷基）胺。基于ETH 1907的微电极在优化的膜组成下，在pH 2至9范围内呈现线性动态响应函数，且该函数扩展到了酸性范围，响应时间小于或等于5秒，对于尖端直径约为1微米的电极，其电阻约为35 GΩ。这使得该电极适用于正常生理细胞内pH值以及酸性生理介质中的测量。本文还展示了使用这种微电极对分离灌注的蛙肾近端小管细胞进行的测量。
