School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, PR China; State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070, PR China; School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, PR China.
School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, PR China; State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070, PR China.
Bioelectrochemistry. 2021 Aug;140:107750. doi: 10.1016/j.bioelechem.2021.107750. Epub 2021 Jan 30.
Tissue engineering scaffolds made of biocompatible polymers are promising alternatives for nerve reparation. For this application, cell proliferation will be speeded up by electrostimulation, which required electrically-conductive materials. Here, a biomimicking scaffold with optimized conductivity was developed from electrospun polyacrylonitrile/electrically-conductive polyaniline (PAN/PANI) nanofibers doped with Ni nanoparticles. PAN/PANI/Ni was biocompatible for Schwann cells and exhibited a suitable tensile strength and wettability for cell proliferation. When compared with unmodified PAN/PANI, the electrical conductivity of PAN/PANI/Ni was 6.4 fold higher. Without electrostimulation, PAN/PANI and PAN/PANI/Ni exhibited similar Schwann cells' proliferation rates. Upon electrostimulation at 100 mV cm for one hour per day over five days, PAN/PANI/Ni accelerated Schwann cells' proliferation 2.1 times compared to PAN/PANI. These results demonstrate the importance of expanding the electrical conductivity of the tissue engineering scaffold to ensure optimal electrostimulation of nerve cell growth. Additionally, this study describes a straightforward approach to modulate the electrical conductivity of polymeric materials via the addition of Ni nanoparticles that can be applied to different biomimicking scaffolds for nerve healing.
由生物相容性聚合物制成的组织工程支架是修复神经的有前途的替代品。为此，细胞增殖将通过电刺激加速，这需要导电材料。在这里，从掺杂有 Ni 纳米粒子的静电纺丝聚丙烯腈/导电聚苯胺 (PAN/PANI) 纳米纤维中开发出了一种仿生支架，其具有优化的导电性。PAN/PANI/Ni 对雪旺细胞具有生物相容性，表现出适合细胞增殖的适当拉伸强度和润湿性。与未改性的 PAN/PANI 相比，PAN/PANI/Ni 的电导率高 6.4 倍。在没有电刺激的情况下，PAN/PANI 和 PAN/PANI/Ni 表现出相似的雪旺细胞增殖率。在每天电刺激 100 mV cm 持续 5 天，1 小时后，与 PAN/PANI 相比，PAN/PANI/Ni 可使雪旺细胞增殖加速 2.1 倍。这些结果表明，扩大组织工程支架的导电性对于确保对神经细胞生长的最佳电刺激至关重要。此外，本研究通过添加 Ni 纳米粒子来调节聚合物材料的电导率，这是一种简单的方法，可应用于不同的仿生支架以用于神经修复。
