Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Life Science and Bioprocesses, Fraunhofer Institute for Applied Polymer Research IAP, Geiselbergstraße 68, 14476, Potsdam-Golm, Germany.
Appl Biochem Biotechnol. 2023 Dec;195(12):7638-7651. doi: 10.1007/s12010-023-04526-6. Epub 2023 Apr 18.
Electroconductive scaffolds based on polyaniline (PANi)/polyacrylonitrile (PAN) were fabricated and surface-functionalized by carboxymethyl chitosan (CMC) as efficient scaffolds for nerve tissue regeneration. The results of scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, and water contact angle measurement approved the successful fabrication of CMC-functionalized PANi/PAN-based scaffolds. Human adipose-derived mesenchymal stem cells (hADMSCs) were cultured on the scaffolds for 10 d in the presence or absence of β-carotene (βC, 20 µM) as a natural neural differentiation agent. The MTT and SEM results confirmed the attachment and proliferation of hADMSCs on the scaffolds. The expression of MAP2 at the mRNA and protein levels showed the synergic neurogenic induction effect of CMC-functionalization and βC for hADMSCs on the scaffolds. The CMC-functionalized nanofibrous PANi/PAN-based scaffolds are potential candidates for nerve tissue engineering.
基于聚苯胺（PANI）/聚丙烯腈（PAN）的导电支架通过羧甲基壳聚糖（CMC）进行表面功能化，作为用于神经组织再生的有效支架。扫描电子显微镜（SEM）、傅里叶变换红外（FTIR）光谱和水接触角测量的结果证实了成功制备了 CMC 功能化的基于 PANi/PAN 的支架。在存在或不存在β-胡萝卜素（βC，20 μM）作为天然神经分化剂的情况下，将人脂肪来源间充质干细胞（hADMSCs）在支架上培养 10 天。MTT 和 SEM 结果证实了 hADMSCs 在支架上的附着和增殖。MAP2 在 mRNA 和蛋白质水平上的表达表明 CMC 功能化和βC 对支架上的 hADMSCs 具有协同的神经诱导作用。CMC 功能化的纳米纤维 PANi/PAN 基支架是神经组织工程的潜在候选材料。
