Meyer J S, Coplin M D
St. Luke's Hospital, Chesterfield, Missouri 63017.
Am J Clin Pathol. 1988 May;89(5):586-95. doi: 10.1093/ajcp/89.5.586.
The results of flow cytometry were compared with the tritiated thymidine labeling index (TLI) in 314 tumors studied in one laboratory and 163 tumors from a second laboratory, a total of 477, including 306 breast carcinomas. Flow cytometric DNA measurements were obtained on mechanically dissociated fresh tissue stained with propidium iodide or DAPI. Spearman's rank order correlation coefficients (r) for TLI versus flow cytometric S-phase measurement (%S) were 0.38 to 0.55 (P less than 0.001) for diploid DNA index (DNAI) and 0.59 and 0.67 (P less than 0.001) for aneuploid DNAI. The correlations were similar for breast and nonmammary tumors. No correlation of DNAI with TLI or %S was observed for aneuploid tumors, although the aneuploid tumors had significantly higher TLI and %S than diploid tumors (P less than 0.001). Mean TLI for the breast carcinomas was 4.6% and 3.6% for diploid tumors in Series 1 and 2, respectively; for aneuploid tumors, 9.9% and 9.3%. Respective mean %S for diploid breast carcinomas was 6.8% and 7.4%; for aneuploid breast carcinomas, it was 13.6% and 14.5%. The excess of %S over TLI was greatest for tumors with low TLI and appeared to result largely from debris in DNA histograms. TLI, %S and DNAI each predicted both absolute survival and relapse-free survival of breast carcinoma patients.
在一个实验室研究的314个肿瘤以及另一个实验室的163个肿瘤（共477个，包括306个乳腺癌）中，将流式细胞术的结果与氚标记胸腺嘧啶核苷标记指数（TLI）进行了比较。流式细胞术DNA测量是在机械解离并用碘化丙啶或DAPI染色的新鲜组织上进行的。对于二倍体DNA指数（DNAI），TLI与流式细胞术S期测量值（%S）的Spearman等级相关系数（r）为0.38至0.55（P小于0.001），对于非整倍体DNAI为0.59和0.67（P小于0.001）。乳腺癌和非乳腺肿瘤的相关性相似。对于非整倍体肿瘤，未观察到DNAI与TLI或%S的相关性，尽管非整倍体肿瘤的TLI和%S显著高于二倍体肿瘤（P小于0.001）。系列1和系列2中二倍体肿瘤的乳腺癌平均TLI分别为4.6%和3.6%；非整倍体肿瘤为9.9%和9.3%。二倍体乳腺癌的平均%S分别为6.8%和7.4%；非整倍体乳腺癌为13.6%和14.5%。对于TLI较低的肿瘤，%S超过TLI的幅度最大，这似乎主要是由于DNA直方图中的碎片所致。TLI、%S和DNAI均能预测乳腺癌患者的绝对生存率和无复发生存率。
