Zhou Yimin, Chang Cong, Liu Zuhao, Zhao Qiuling, Xu Qingni, Li Chaohua, Chen Yuqi, Zhang Yueli, Lu Bo
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, PR China.
College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430070, PR China.
Langmuir. 2021 Mar 2;37(8):2619-2628. doi: 10.1021/acs.langmuir.0c03250. Epub 2021 Feb 14.
Hollow mesoporous silica nanoparticles (HMSNs) served as nanocarriers for transporting doxorubicin hydrochloride (DOX) and indocyanine green (ICG) and were incorporated into a pH-sensitive targeted drug delivery system (DDS). Boronate ester bonds were employed to link HMSNs and dopamine-modified hyaluronic acid (DA-HA), which acted as both the "gatekeeper" and targeting agents (HMSNs-B-HA). Well-dispersed HMSNs-B-HA with a diameter of about 170 nm was successfully constructed. The conclusion was drawn from the in vitro drug release experiment that ICG and DOX (ID) co-loaded nanoparticles (ID@HMSNs-B-HA) with high drug loading efficiency could sustain drug release under acidic conditions. More importantly, in vitro cell experiments perfectly showed that ID@HMSNs-B-HA could well inhibit murine mammary carcinoma (4T1) cells via chemotherapy combined with photodynamic therapy and accurately target 4 T1 cells. In summary, all test results sufficiently demonstrated that the prepared ID@HMSNs-B-HA was a promising nano-DDS for cancer photodynamic combined with chemotherapy.
中空介孔二氧化硅纳米粒子（HMSNs）作为纳米载体用于运输盐酸多柔比星（DOX）和吲哚菁绿（ICG），并被整合到一种pH敏感的靶向给药系统（DDS）中。硼酸酯键用于连接HMSNs和多巴胺修饰的透明质酸（DA-HA），后者同时充当“守门人”和靶向剂（HMSNs-B-HA）。成功构建了直径约为170 nm且分散良好的HMSNs-B-HA。体外药物释放实验得出的结论是，具有高载药效率的ICG和DOX（ID）共负载纳米粒子（ID@HMSNs-B-HA）在酸性条件下能够持续释放药物。更重要的是，体外细胞实验完美地表明，ID@HMSNs-B-HA通过化疗联合光动力疗法能够很好地抑制小鼠乳腺癌（4T1）细胞，并能准确靶向4T1细胞。总之，所有测试结果充分证明，所制备的ID@HMSNs-B-HA是一种用于癌症光动力联合化疗的有前景的纳米给药系统。
