Department of Orthopaedics, Joint Centre of Translational Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325035, P. R. China.
Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325011, P. R. China.
J Mater Chem B. 2021 Mar 4;9(8):2033-2041. doi: 10.1039/d0tb02588g.
Xenogeneic bones are potential templates for bone regeneration. In this study, decellularized porcine bone powder with attenuated immunogenicity was incorporated into a photocurable hydrogel, gelatin methacryloyl (GelMA), to obtain scaffolds with good mechanical properties for bone tissue engineering. The decellularized bone powder (DCB)-GelMA hybrid scaffolds had higher compressive strength and stiffness values when the DCB content was increased. In vitro evaluations revealed the biocompatibility of these scaffolds. The scaffolds could induce human bone marrow mesenchymal stem cells (hMSCs) to undergo osteogenic differentiation even in the absence of an induction medium. The efficiency of the scaffolds for bone regeneration applications was further evaluated using an in vivo cranial bone defect model in rats. Micro-CT images showed that the hybrid scaffolds with 20% DCB content had the best effect in promoting new bone regeneration. Thus, it was concluded that the DCB-GelMA hybrid scaffolds have high potential in bone tissue engineering applications.
异种骨是骨再生的潜在模板。在这项研究中，脱细胞猪骨粉的免疫原性被减弱，并与光固化水凝胶明胶甲基丙烯酰（GelMA）结合，以获得具有良好机械性能的用于骨组织工程的支架。当 DCB 含量增加时，脱细胞骨粉（DCB）-GelMA 杂化支架具有更高的抗压强度和刚度值。体外评估显示了这些支架的生物相容性。支架即使在没有诱导培养基的情况下，也能诱导人骨髓间充质干细胞（hMSCs）向成骨细胞分化。使用大鼠颅骨缺损模型进一步评估了支架在骨再生应用中的效率。微 CT 图像显示，含有 20% DCB 的杂化支架在促进新骨再生方面效果最佳。因此，可以得出结论，DCB-GelMA 杂化支架在骨组织工程应用中具有很大的潜力。
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