Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara City Hospital, Ankara, Turkey.
Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara City Hospital, Ankara, Turkey.
Free Radic Biol Med. 2021 Apr;166:11-17. doi: 10.1016/j.freeradbiomed.2021.02.009. Epub 2021 Feb 13.
Thiol-disulphide homeostasis (TDH) is a new parameter indicating oxidative stress that plays a role in the pathogenesis of various clinical disorders. Our study planned to investigate TDH in COVID-19 patients. Age and gender-matched healthy subjects (n = 70) and COVID-19 patients (n = 144) were included in the study. In addition to the routine laboratory parameters of the groups, their native thiol (NT), total thiol (TT) and disulphide levels were measured. Primarily, we compared COVID-19 patients to the healthy control group for inflammatory parameters, NT, TT and disulphide levels. Then, COVID-19 patients were divided into two groups according to the severity of the disease as mild to moderate and severe COVID-19, and the three groups were compared with each other. Predictive value of thiol parameters in the diagnosis of COVID-19 and in the determining its severity, and its correlation with presence and duration of symptoms were investigated. Severe COVID-19 patients had lower NT and TT levels compared with healthy controls and mild to moderate patients (P < 0.001 for both). The results of ROC analysis show that the greatest AUC was IL-6 and NT (AUC = 0.97, AUC = 0.96, respectively) between control and COVID-19 patients, while it was CRP and NT (AUC = 0.85, AUC = 0.83) between mild to moderate and severe patients. A negative correlation was found between duration of symptoms of dyspnoea, cough, fever, and sore throat and NT (r = -0.45, P = 0.017, r = -0.418, P < 0.001, r = -0.131, P = 0.084, r = -0.452, P = 0.040, respectively). NT and TT levels have a strong predictive value in the diagnosis of COVID-19 and in determining disease severity. Our results support that changing TDH parameters appears to have an important role in disease pathogenesis and it can be used in clinical management of patients.
硫醇-二硫键稳态（TDH）是一种新的氧化应激参数，在各种临床疾病的发病机制中起作用。我们的研究计划调查 COVID-19 患者的 TDH。将年龄和性别匹配的健康受试者（n=70）和 COVID-19 患者（n=144）纳入研究。除了对两组的常规实验室参数进行测量外，还测量了他们的天然硫醇（NT）、总硫醇（TT）和二硫键水平。首先，我们将 COVID-19 患者与健康对照组进行比较，比较炎症参数、NT、TT 和二硫键水平。然后，根据疾病的严重程度将 COVID-19 患者分为轻度至中度和重度 COVID-19 两组，并将三组相互比较。研究了硫醇参数在 COVID-19 诊断中的预测价值及其严重程度，并研究了与症状的存在和持续时间的相关性。与轻度至中度患者相比，严重 COVID-19 患者的 NT 和 TT 水平较低（均 P<0.001）。ROC 分析结果表明，在控制组和 COVID-19 患者之间，IL-6 和 NT 的 AUC 最大（AUC=0.97、AUC=0.96），而在轻度至中度和重度患者之间，CRP 和 NT 的 AUC 最大（AUC=0.85、AUC=0.83）。呼吸困难、咳嗽、发热和喉咙痛症状持续时间与 NT 呈负相关（r=-0.45，P=0.017，r=-0.418，P<0.001，r=-0.131，P=0.084，r=-0.452，P=0.040）。NT 和 TT 水平在 COVID-19 的诊断和疾病严重程度的确定方面具有很强的预测价值。我们的研究结果支持 TDH 参数的变化似乎在疾病发病机制中起着重要作用，可用于患者的临床管理。
