Department of Chemical and Biological Engineering and Center for Synthetic Biology, Northwestern University, Evanston, IL 60208, USA.
Chem Commun (Camb). 2021 Mar 14;57(21):2661-2664. doi: 10.1039/d0cc07740b. Epub 2021 Feb 16.
We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosome in vitro through genetic code reprogramming.
我们报告了一系列荧光氨基酸（FAAs）的设计、化学合成以及 Flexizyme 催化的转移 RNA（tRNA）酰化。荧光团包括芘、香豆素、硝基苯并二氮杂卓和荧光素变体。我们进一步通过遗传密码重编程证明了核糖体在体外将这些 FAAs 特异性地掺入到肽中。
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