Innovative Manufacturing, Mechatronics and Sports Lab (iMAMS), Faculty of Manufacturing and Mechatronics Engineering Technology, University Malaysia Pahang, 26600, Pekan, Pahang, Malaysia.
University College London, Gower St, Bloomsbury, London, WC1E 6BT, UK.
Microb Cell Fact. 2021 Feb 17;20(1):46. doi: 10.1186/s12934-020-01478-y.
With the long-term goal of developing an ultra-sensitive microcantilever-based biosensor for versatile biomarker detection, new controlled bioreceptor-analytes systems are being explored to overcome the disadvantages of conventional ones. Gold (Au) microwires have been used as a probe to overcome the tolerance problem that occurs in response to changes in environmental conditions. However, the cytotoxicity of Au microwires is still unclear. Here, we examined the cytotoxicity of Au microwires systems using both commercial and as-synthesised Au microwires. In vitro experiments show that commercial Au microwires with an average quoted length of 5.6 µm are highly toxic against Gram-negative Escherichia coli (E. coli) at 50 µg/mL. However, this toxicity is due to the presence of CTAB surfactant not by the microwires. Conversely, the as-synthesised Au microwires show non-cytotoxicity even at the maximum viable concentration (330 µg/mL). These findings may lead to the development of potentially life-saving cytotoxicity-free biosensors for an early diagnostic of potential diseases.
为了长期开发一种超灵敏的基于微悬臂梁的生物传感器，用于各种生物标志物的检测，我们正在探索新的、受控制的生物受体-分析物系统，以克服传统系统的缺点。金（Au）微丝已被用作探针，以克服在响应环境条件变化时出现的耐受性问题。然而，Au 微丝的细胞毒性仍不清楚。在这里，我们使用商业和合成的 Au 微丝研究了 Au 微丝系统的细胞毒性。体外实验表明，平均长度为 5.6 µm 的商业 Au 微丝在 50 µg/mL 时对革兰氏阴性大肠杆菌（E. coli）具有高度毒性。然而，这种毒性是由于存在 CTAB 表面活性剂而不是微丝造成的。相反，即使在最大存活率浓度（330 µg/mL）下，合成的 Au 微丝也显示出非细胞毒性。这些发现可能会导致开发出潜在的、无细胞毒性的生物传感器，用于对潜在疾病进行早期诊断。
