Singh Chandrashekhar, Anand Sumit Kumar, Tiwari Kavindra Nath, Mishra Sunil Kumar, Kakkar Poonam
Department of Botany, MMV, Banaras Hindu University, Varanasi, 221005 Uttar Pradesh India.
CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan 31, Mahatma Gandhi Marg, Lucknow, 226001 Uttar Pradesh India.
3 Biotech. 2021 Mar;11(3):115. doi: 10.1007/s13205-021-02654-6. Epub 2021 Feb 5.
L. (Lamiaceae) is a medicinal plant, widely distributed in the tropical and subtropical regions and commonly used in traditional medicine. The current study was focused to evaluate the cytotoxic potential of aqueous extract of root of (AEPS) against human hepatoblastoma cancer cell line (Hep G2).The yield of the dried extract was 5.8% and used for further studies.Cytotoxic potential of AEPS was analyzed by MTT assay, which exhibits IC value 1000 µg/mL after 48 h incubation. Hoechst and AO/EtBr staining, ROS measurement, mitochondrial membrane potential, clonogenic and wound healing assays also confirmed the cytotoxic efficacy of AEPS in dose and time-dependent manner. UPLC-Q-TOF-MS/MS analysis of AEPS confirmed the presence of 12polyphenolic compounds, namely 4-hydroxy-3-methoxycinnamic acid, linarin, peonidin-3,5-O-di-beta-glucopyranoside, diosmin, trans-cinnamic acid, daidzein, saponarin, homoorietin, acacetin, sarsasapogenin, phytol and sissotrin. The cytotoxic potential of AEPS might due to presence of biologically active polyphenolic compounds.
唇形科植物是一种药用植物，广泛分布于热带和亚热带地区，常用于传统医学。当前的研究聚焦于评估[唇形科植物名称]根的水提取物（AEPS）对人肝癌细胞系（Hep G2）的细胞毒性潜力。干燥提取物的得率为5.8%，并用于进一步研究。通过MTT法分析AEPS的细胞毒性潜力，在孵育48小时后其IC值为1000μg/mL。Hoechst和AO/EtBr染色、活性氧测量、线粒体膜电位、克隆形成和伤口愈合试验也以剂量和时间依赖性方式证实了AEPS的细胞毒性功效。对AEPS进行超高效液相色谱-四极杆-飞行时间串联质谱（UPLC-Q-TOF-MS/MS）分析，证实存在12种多酚类化合物，即4-羟基-3-甲氧基肉桂酸、蒙花苷、芍药色素-3,5-O-二-β-葡萄糖苷、香叶木素、反式肉桂酸、大豆苷元、肥皂草苷、高圣草素、刺槐素、菝葜皂苷元、叶绿醇和异鼠李素。AEPS的细胞毒性潜力可能归因于生物活性多酚类化合物的存在。
