评估苏丹患者单纯性骨折患者的血浆 BMP-2、BMP-7、BMP-10、维生素 D 和 TGFβ1。

Assessment of plasma BMP-2, BMP-7, BMP-10, vitamin D, and TGF β1 in simple fractures among Sudanese patients.

机构信息

Department of Biochemistry, Faculty of Medicine-University of Khartoum, Khartoum, Sudan.

Department of Immunology at Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.

出版信息

PLoS One. 2021 Feb 19;16(2):e0247472. doi: 10.1371/journal.pone.0247472. eCollection 2021.

DOI:10.1371/journal.pone.0247472

PMID:33606840

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7895376/

Abstract

BACKGROUND

Bone morphogenetic proteins (BMP) are multifunctional proteins. They work as cytokines regulating osteogenesis during fracture healing process. The objectives of this study were to assess changes in BMPs during fracture and their correlations to Fracture's healing.

METHODS

Case-Control hospital-based study conducted from January 2018 to January 2019. Demographic data, anthropometric measurements, and blood samples were collected from patients and controls (18-65 years old). Plasma concentrations of selected BMPs and vitamin D were measured using quantitative enzyme linked immunosorbent assay (ELISA). SPSS version 25 was used to calculate frequencies, Pearson correlation tests, chi-square and unpaired t-test.

RESULTS

Sixty-five patients with fractures and Sixty-five controls were studied. Means of plasma concentrations were (TGFβ1 = 21.07 ng/ml ±8.49 and 19.8 ng/ml ±7.2) (BMP-2 = 76.3 pg/ml ± 156.6 and 55.5 ng/ml ± 127.9) (BMP-7 = 13.02 pg/ml ±43.5 and 64.6pg/ml ±250) (BMP-10 = 8.14 pg/ml ±12.7 and 5.48 pg/ml ±11.3) (Vitamin D mean was 24.94 ng/ml ±13.2 and 26.2 ng/ml ±11.6) in patients and controls, respectively. Forty-five subjects were enrolled into follow up study: 30 males, 15 females. Healing time mean was 4.13± 2.6 months. No significant correlation between BMP-2/BMP-7 with healing time.

CONCLUSIONS

BMP-7 was significantly lowers in the plasma of patients that controls (P = 0.042). Low Vitamin D was observed among Sudanese participants.

摘要

背景

骨形态发生蛋白（BMP）是一种多功能蛋白，在骨折愈合过程中作为调节成骨的细胞因子发挥作用。本研究旨在评估骨折过程中 BMP 的变化及其与骨折愈合的相关性。

方法

这是一项 2018 年 1 月至 2019 年 1 月在医院进行的病例对照研究。从患者和对照组（18-65 岁）中收集人口统计学数据、人体测量学测量值和血液样本。采用定量酶联免疫吸附试验（ELISA）测量选定 BMP 和维生素 D 的血浆浓度。使用 SPSS 版本 25 计算频率、皮尔逊相关检验、卡方检验和独立样本 t 检验。

结果

共研究了 65 例骨折患者和 65 例对照组患者。患者和对照组的血浆浓度平均值分别为（TGFβ1=21.07ng/ml±8.49 和 19.8ng/ml±7.2）（BMP-2=76.3pg/ml±156.6 和 55.5ng/ml±127.9）（BMP-7=13.02pg/ml±43.5 和 64.6pg/ml±250）（BMP-10=8.14pg/ml±12.7 和 5.48pg/ml±11.3）（维生素 D 平均值分别为 24.94ng/ml±13.2 和 26.2ng/ml±11.6）。45 名患者纳入随访研究：30 名男性，15 名女性。愈合时间的平均值为 4.13±2.6 个月。BMP-2/BMP-7 与愈合时间之间无显著相关性。