Schmid B J, Perry H E, Idle J R
Department of Pharmacology and Toxicology, St. Mary's Hospital Medical School, London, U.K.
J Chromatogr. 1988 Mar 4;425(1):107-19. doi: 10.1016/0378-4347(88)80011-2.
A sensitive, efficient, linear and reproducible capillary gas chromatographic method with electron-capture detection was developed for the quantitation of nifedipine and its primary metabolite M-I in plasma together with the urinary and principal metabolites M-II and M-III. On-column, rather than split-splitless, injection was employed to obviate oxidative degradation of nifedipine to M-I. The photosensitivity of nifedipine was re-examined under laboratory conditions and nifedipine was found to have a half-life in excess of two days when amber glassware and darkroom manipulations under red light were used. The method can determine nifedipine and its metabolites in plasma and urine after a single oral dose of 5 mg and can be applied to measure M-I production by human liver microsomes.
建立了一种灵敏、高效、线性且可重现的毛细管气相色谱法，采用电子捕获检测，用于定量测定血浆中硝苯地平及其主要代谢物M-I以及尿液中的主要代谢物M-II和M-III。采用柱上进样而非分流/不分流进样，以避免硝苯地平氧化降解为M-I。在实验室条件下重新研究了硝苯地平的光敏性，发现当使用琥珀色玻璃器皿并在红光下于暗室中操作时，硝苯地平的半衰期超过两天。该方法可在单次口服5毫克后测定血浆和尿液中的硝苯地平及其代谢物，可用于测定人肝微粒体产生M-I的情况。
