Li Min, Geng Rongxin, Li Chen, Meng Fantao, Zhao Hongwei, Liu Jing, Dai Juanjuan, Wang Xuezhen
Department of Neurology, Binzhou Medical University Hospital, No. 661 Huanghe 2nd Road, Binzhou, Shandong, 256603, China.
Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430000, China.
Transl Neurosci. 2021 Feb 5;12(1):83-95. doi: 10.1515/tnsci-2021-0009. eCollection 2021 Jan 1.
Alzheimer's disease (AD), the most common type of dementia, is a neurodegenerative disorder with a hidden onset, including difficult early detection and diagnosis. Nevertheless, the new crucial biomarkers for the diagnosis and pathogenesis of AD need to be explored further. Here, the common differentially expressed genes (DEGs) were identified through a comprehensive analysis of gene expression profiles from the Gene Expression Omnibus (GEO) database. Furthermore, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that these DEGs were mainly associated with biological processes, cellular components, and molecular functions, which are involved in multiple cellular functions. Next, we found that 9 of the 24 genes showed the same regulatory changes in the blood of patients with AD compared to those in the GEO database, and 2 of the 24 genes showed a significant correlation with Montreal Cognitive Assessment scores. Finally, we determined that mice with AD and elderly mice had the same regulatory changes in the identified DEGs in both the blood and hippocampus. Our study identified several potential core biomarkers of AD and aging, which could contribute to the early detection, differential diagnosis, treatment, and pathological analysis of AD.
阿尔茨海默病（AD）是最常见的痴呆类型，是一种起病隐匿的神经退行性疾病，包括早期难以检测和诊断。然而，仍需进一步探索用于AD诊断和发病机制的新的关键生物标志物。在此，通过对来自基因表达综合数据库（GEO）的基因表达谱进行全面分析，鉴定出常见的差异表达基因（DEG）。此外，基因本体论和京都基因与基因组百科全书通路分析表明，这些DEG主要与生物过程、细胞成分和分子功能相关，涉及多种细胞功能。接下来，我们发现24个基因中有9个在AD患者血液中的调控变化与GEO数据库中的一致，24个基因中有2个与蒙特利尔认知评估得分显著相关。最后，我们确定AD小鼠和老年小鼠在血液和海马中鉴定出的DEG具有相同的调控变化。我们的研究鉴定出了几种AD和衰老的潜在核心生物标志物，这可能有助于AD的早期检测、鉴别诊断、治疗和病理分析。
