Phasics, Bâtiment Explorer, Espace Technologique, Route de l'Orme des Merisiers, 91190, St Aubin, France.
Transporter in Imaging and Radiotherapy in Oncology (TIRO), Institut des Sciences et Biotechnologies du Vivant Frédéric Joliot, CEA, School of Medicine, 28 Av de Valombrose, 06107, Nice, France.
Sci Rep. 2021 Feb 24;11(1):4409. doi: 10.1038/s41598-021-83537-x.
We present here a label-free development based on preexisting Quantitative Phase Imaging (QPI) that allows non-invasive live monitoring of both individual cells and cell populations. Growth, death, effect of toxic compounds are quantified under visible light with a standard inverted microscope. We show that considering the global biomass of a cell population is a more robust and accurate method to assess its growth parameters in comparison to compiling individually segmented cells. This is especially true for confluent conditions. This method expands the use of light microscopy in answering biological questions concerning live cell populations even at high density. In contrast to labeling or lysis of cells this method does not alter the cells and could be useful in high-throughput screening and toxicity studies.
我们在这里提出了一种基于已有定量相位成像（QPI）的无标记开发，它允许对单个细胞和细胞群体进行非侵入性的实时监测。在可见光下，使用标准倒置显微镜定量测量细胞的生长、死亡和有毒化合物的影响。我们表明，与单独分割细胞相比，考虑细胞群体的整体生物量是评估其生长参数更稳健和准确的方法。对于细胞达到汇合状态时尤其如此。这种方法扩展了使用光学显微镜来回答有关活细胞群体的生物学问题的应用，即使在高细胞密度下也是如此。与细胞标记或裂解不同，该方法不会改变细胞，并且在高通量筛选和毒性研究中可能很有用。
