Habiba Ume, Nisar Jaweria, Choohan Muhammad Akram, Shah Syed Muhammad Ali, Nisar Zonaira, Mustafa Imtiaz
Department of Microbiology and Molecular genetics, The Women University Multan, Multan, Punjab, Pakistan.
University College of Conventional Medicine, Islamia University of Bahawalpur, Bahawalpur, Punjab, Pakistan.
Dose Response. 2021 Feb 11;19(1):1559325821992239. doi: 10.1177/1559325821992239. eCollection 2021 Jan-Mar.
Medicinal plants are gaining popularity over synthetic medicines because antibiotic resistance demands the alternative source of medication. In the present research, the crude protein extraction of 4 medicinal plants and was carried out. Crude protein extraction was done by 2 different buffers i.e. Tris NaCl buffer and PBS buffer. Protein confirmation was done by Bradford assay in the spectrophotometer. Antibacterial potential was checked and compared against and Antibacterial assay was performed by disc diffusion method, agar well method and zones of inhibition were calculated. The study results indicated that Tris NaCl extracts' antimicrobial potential is higher than that of the PBS buffer. On disc diffusion method the Tris NaCl buffer extracts of showed maximum zone of inhibition 11 mm and 9 mm against and respectively and control showed maximum zone of inhibition 26 mm against showed maximum zone of inhibition of 7 mm against while and didn't show the any antibacterial activity. On the other hand, Protein extracts from PBS buffer didn't show zone of inhibition against any bacteria. Only showed minute zone of inhibition against . On well diffusion method, Tris NaCl protein extract showed the maximum zone of inhibition 20 mm and 18 mm against and respectively. While PBS protein extract showed the maximum zone of inhibition 19 mm and 17 mm against and The results revealed that the protein extract of can be used tosynthesize antimicrobial drugs to treat the bacterial infections.
由于抗生素耐药性使得需要替代药物来源，药用植物比合成药物越来越受欢迎。在本研究中，对4种药用植物进行了粗蛋白提取。粗蛋白提取通过两种不同的缓冲液进行，即Tris NaCl缓冲液和PBS缓冲液。通过分光光度计中的Bradford测定法进行蛋白质确认。检测了抗菌潜力并与……进行比较，通过纸片扩散法进行抗菌测定，计算琼脂孔法和抑菌圈。研究结果表明，Tris NaCl提取物的抗菌潜力高于PBS缓冲液。在纸片扩散法中，……的Tris NaCl缓冲液提取物对……和……分别显示出最大抑菌圈11毫米和9毫米，对照对……显示出最大抑菌圈26毫米，对……显示出最大抑菌圈7毫米，而……和……未显示出任何抗菌活性。另一方面，PBS缓冲液的蛋白质提取物对任何细菌均未显示出抑菌圈。只有……对……显示出微小的抑菌圈。在孔扩散法中，Tris NaCl蛋白提取物对……和……分别显示出最大抑菌圈20毫米和18毫米。而PBS蛋白提取物对……和……分别显示出最大抑菌圈19毫米和17毫米。结果表明，……的蛋白质提取物可用于合成抗菌药物以治疗细菌感染。
