Antibacterial Activity of Tris NaCl and PBS Buffer Protein Extract of , , and Against Bacterial Strains.

Medicinal plants are gaining popularity over synthetic medicines because antibiotic resistance demands the alternative source of medication. In the present research, the crude protein extraction of 4 medicinal plants and was carried out. Crude protein extraction was done by 2 different buffers i.e. Tris NaCl buffer and PBS buffer. Protein confirmation was done by Bradford assay in the spectrophotometer. Antibacterial potential was checked and compared against and Antibacterial assay was performed by disc diffusion method, agar well method and zones of inhibition were calculated. The study results indicated that Tris NaCl extracts' antimicrobial potential is higher than that of the PBS buffer. On disc diffusion method the Tris NaCl buffer extracts of showed maximum zone of inhibition 11 mm and 9 mm against and respectively and control showed maximum zone of inhibition 26 mm against showed maximum zone of inhibition of 7 mm against while and didn't show the any antibacterial activity. On the other hand, Protein extracts from PBS buffer didn't show zone of inhibition against any bacteria. Only showed minute zone of inhibition against . On well diffusion method, Tris NaCl protein extract showed the maximum zone of inhibition 20 mm and 18 mm against and respectively. While PBS protein extract showed the maximum zone of inhibition 19 mm and 17 mm against and The results revealed that the protein extract of can be used tosynthesize antimicrobial drugs to treat the bacterial infections.