Lv Kangning, Wang Lei, He Xiaoli, Li Wenjun, Han Lei, Qin Song
School of Ocean, Yantai University, Yantai, China.
Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China.
Front Bioeng Biotechnol. 2022 Feb 14;9:792344. doi: 10.3389/fbioe.2021.792344. eCollection 2021.
Extracellular matrix (ECM) material with good biological activity is essential to simulate cell growth microenvironment, induce cell infiltration and angiogenesis, and promote the repair of large area acute skin wound. In this study, tilapia skin acellular dermal matrix (TADM) was prepared to simulate ECM microenvironment, which can promote substantial area acute wound healing in rats. The main component of TADM is type I collagen, which has good physical and chemical properties, biological activity and cell adhesion. TADM is a form of biomaterial with low immunogenicity, low risk of prion infection and lower economic cost than other related materials such as mammalian collagen biomaterials. Our results show that TADM can guide cell infiltration, angiogenesis, regulate the expression and secretion of inflammatory and skin repair correlated factors to promote tissue healing.
具有良好生物活性的细胞外基质(ECM)材料对于模拟细胞生长微环境、诱导细胞浸润和血管生成以及促进大面积急性皮肤伤口的修复至关重要。在本研究中,制备了罗非鱼皮脱细胞真皮基质(TADM)以模拟ECM微环境,其可促进大鼠大面积急性伤口愈合。TADM的主要成分是I型胶原蛋白,其具有良好的物理和化学性质、生物活性以及细胞粘附性。TADM是一种生物材料形式,具有低免疫原性、低朊病毒感染风险且比其他相关材料(如哺乳动物胶原蛋白生物材料)具有更低的经济成本。我们的结果表明,TADM可引导细胞浸润、血管生成,调节炎症和皮肤修复相关因子的表达与分泌以促进组织愈合。