用于琥珀酸生物合成的丙酰辅酶A羧化酶的定向进化
Directed Evolution of Propionyl-CoA Carboxylase for Succinate Biosynthesis.
作者信息
Liu Yuwan, Jiang Huifeng
机构信息
Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China; National Technology Innovation Center of Synthetic Biology, Tianjin, 300308, China.
Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China; National Technology Innovation Center of Synthetic Biology, Tianjin, 300308, China.
出版信息
Trends Biotechnol. 2021 Apr;39(4):330-331. doi: 10.1016/j.tibtech.2021.02.006. Epub 2021 Feb 22.
Due to low carboxylase activity, CO biotransformation is challenging to achieve using natural CO fixation pathways. Liu et al. have improved the activity of propionyl-CoA carboxylase (PCC) 94-fold, enabling the efficient synthesis of succinate from acetyl-CoA and paving the way for CO assimilation via the 3-hydroxypropionate (3-HP) bicycle or 3-hydroxypropionate/4-hydroxybutyrate (3-HP/4-HB) cycle.
由于羧化酶活性较低，利用天然的一氧化碳固定途径实现一氧化碳生物转化具有挑战性。刘等人将丙酰辅酶A羧化酶（PCC）的活性提高了94倍，实现了从乙酰辅酶A高效合成琥珀酸，为通过3-羟基丙酸（3-HP）循环或3-羟基丙酸/4-羟基丁酸（3-HP/4-HB）循环进行一氧化碳同化铺平了道路。
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