Wang Mi, Wang Bo, Wang Shanshan, Lu Han, Wu Hao, Ding Manyi, Ying Linlin, Mao Yanjun, Li Yao
Institute of Animal Nutrition, Northeast Agricultural University, Harbin, China.
College of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University, Jinzhou, China.
Front Cell Dev Biol. 2021 Feb 9;9:616219. doi: 10.3389/fcell.2021.616219. eCollection 2021.
The present study was conducted to investigate effects and mechanism of quercetin on lipids metabolism in broilers. 480 AA broilers were randomly allotted to four treatments (0, 0.2, 0.4, and 0.6 g/kg quercetin) for 42 days. Compared with the control, 0.6 g/kg quercetin significantly decreased percentage of abdominal fat ( < 0.05); 0.2, 0.4, and 0.6 g/kg quercetin significantly decreased relative abundance of and ( < 0.05, < 0.05, < 0.01; < 0.01, < 0.01, < 0.01); 0.2 g/kg quercetin significantly increased mRNA expression of PI3K, AMPKα1, AMPKα2, AMPKβ2, LKB1 ( < 0.01, < 0.01, < 0.05, < 0.01, < 0.05), and significantly reduced mRNA expression of SREBP1 and PPARγ ( < 0.01, < 0.05); 0.4 g/kg quercetin significantly increased mRNA expression of LKB1 and PKB ( < 0.05, < 0.01) and significantly reduced mRNA expression of ACC, HMGR, PPARγ, and SREBP1 ( < 0.05, < 0.01, < 0.01, < 0.01); 0.6 g/kg quercetin significantly increased mRNA expression of AMPKγ, LKB1, CPT1, PPARα, PKB ( < 0.01, < 0.01, < 0.01, < 0.05, < 0.05), and significantly reduced the mRNA expression of PI3K, ACC, HMGR, PPARγ, SREBP1 ( < 0.05, < 0.05, < 0.01, < 0.01, < 0.01); 0.2 g/kg quercetin significantly increased protein expression of AMPK ( < 0.01); 0.6 g/kg quercetin significantly increased protein expression of LKB1 ( < 0.01), 0.2 and 0.6 g/kg quercetin significantly increased protein expression of PI3K, PKB, CPT1 ( < 0.05, < 0.01, < 0.05, < 0.01, < 0.01, < 0.01), and significantly reduced protein expression of ACC and SREBP1 ( < 0.01, < 0.01, < 0.01, < 0.01). In conclusion, quercetin improved lipid metabolism by modulating gut microbial and AMPK/PPAR signaling pathway in broilers.
本研究旨在探讨槲皮素对肉鸡脂质代谢的影响及其机制。480只AA肉鸡被随机分为四组（0、0.2、0.4和0.6克/千克槲皮素），为期42天。与对照组相比，0.6克/千克槲皮素显著降低了腹脂百分比（P<0.05）；0.2、0.4和0.6克/千克槲皮素显著降低了……和……的相对丰度（P<0.05，P<0.05，P<0.01；P<0.01，P<0.01，P<0.01）；0.2克/千克槲皮素显著提高了PI3K、AMPKα1、AMPKα2、AMPKβ2、LKB1的mRNA表达（P<0.01，P<0.01，P<0.05，P<0.01，P<0.05），并显著降低了SREBP1和PPARγ的mRNA表达（P<0.01，P<0.05）；0.4克/千克槲皮素显著提高了LKB1和PKB的mRNA表达（P<0.05，P<0.01），并显著降低了ACC、HMGR、PPARγ和SREBP1的mRNA表达（P<0.05，P<0.01，P<0.01，P<0.01）；0.6克/千克槲皮素显著提高了AMPKγ、LKB1、CPT1、PPARα、PKB的mRNA表达（P<0.01，P<0.01，P<0.01，P<0.05，P<0.05），并显著降低了PI3K、ACC、HMGR、PPARγ、SREBP1的mRNA表达（P<0.05，P<0.05，P<0.01，P<0.01，P<0.01）；0.2克/千克槲皮素显著提高了AMPK的蛋白表达（P<0.01）；0.6克/千克槲皮素显著提高了LKB1的蛋白表达（P<0.01），0.2和0.6克/千克槲皮素显著提高了PI3K、PKB、CPT1的蛋白表达（P<0.05，P<0.01，P<0.05，P<0.01，P<0.01，P<0.01），并显著降低了ACC和SREBP1的蛋白表达（P<0.01，P<0.01，P<0.01，P<0.01）。总之，槲皮素通过调节肉鸡肠道微生物群和AMPK/PPAR信号通路改善了脂质代谢。
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