School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore, 637457, Singapore.
The Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Taiyuan, 030032, China.
Adv Mater. 2021 Apr;33(14):e2008061. doi: 10.1002/adma.202008061. Epub 2021 Feb 26.
Cell-membrane-coated nanoparticles (CCNPs) that integrate the biophysiological advantages of cell membranes with the multifunctionalities of synthetic materials hold great promise in cancer immunotherapy. However, strategies have yet to be revealed to further improve their immunotherapeutic efficacy. Herein, a polymer multicellular nanoengager (SPNE) for synergistic second-near-infrared-window (NIR-II) photothermal immunotherapy is reported. The nanoengager consists of an NIR-II absorbing polymer as the photothermal core, which is camouflaged with fused membranes derived from immunologically engineered tumor cells and dendritic cells (DCs) as the cancer vaccine shell. In association with the high accumulation in lymph nodes and tumors, the multicellular engagement ability of the SPNE enables effective cross-interactions among tumor cells, DCs, and T cells, leading to augmented T cell activation relative to bare or tumor-cell-coated nanoparticles. Upon deep-tissue penetrating NIR-II photoirradiation, SPNE eradicates the tumor and induces immunogenic cell death, further eliciting anti-tumor T cell immunity. Such a synergistic photothermal immunotherapeutic effect eventually inhibits tumor growth, prevents metastasis and procures immunological memory. Thus, this study presents a general cell-membrane-coating approach to develop photo-immunotherapeutic agents for cancer therapy.
细胞膜包覆的纳米颗粒（CCNPs）整合了细胞膜的生物物理优势和合成材料的多功能性，在癌症免疫治疗中具有广阔的应用前景。然而，目前还需要进一步的策略来提高其免疫治疗效果。在此，我们报道了一种用于协同近红外二区（NIR-II）光热免疫治疗的聚合物多细胞纳米交联剂（SPNE）。该纳米交联剂由 NIR-II 吸收聚合物作为光热核心组成，表面伪装有源自免疫工程化肿瘤细胞和树突状细胞（DCs）的融合膜作为癌症疫苗外壳。由于在淋巴结和肿瘤中有较高的蓄积，SPNE 的多细胞交联能力能够有效促进肿瘤细胞、DCs 和 T 细胞之间的交叉相互作用，从而导致 T 细胞的激活增强，优于裸纳米颗粒或肿瘤细胞包覆的纳米颗粒。在穿透深层组织的 NIR-II 光照射下，SPNE 能够消灭肿瘤并诱导免疫原性细胞死亡，进一步引发抗肿瘤 T 细胞免疫。这种协同的光热免疫治疗效果最终抑制了肿瘤的生长，防止了转移，并获得了免疫记忆。因此，本研究提出了一种通用的细胞膜包覆方法，用于开发用于癌症治疗的光免疫治疗剂。
