Department of Animal Science, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong Province, PR China.
Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul, Korea.
Vet Med Sci. 2021 Jul;7(4):1369-1378. doi: 10.1002/vms3.462. Epub 2021 Feb 27.
High-temperature environment-induced heat stress (HS) is a hazard environmental element for animals, leading to dramatic changes in physiological and metabolic function. However, the metabolomic-level mechanisms underlying lipid metabolism in liver of slow-growing broilers are still obscure. The present study investigated the effects of HS on hepatic lipidomics in Chinese indigenous slow-growing broilers (Huaixiang chickens). The study includes two treatments, each treatment had 5 replicates with 4 broilers per cage, where a total of 40 eight-week-old female Huaixiang chickens (average initial body weight of 840.75 ± 20.79 g) were randomly divided into normal temperature (NT) and HS groups for 4 weeks, and the broilers of NT and HS groups were exposed to 21.3 ± 1.2℃ and 32.5 ± 1.4℃ respectively. The relative humidity of the two groups was maintained at 55%-70%. The liquid chromatography-mass spectrometry (LC-MS)-based metabolomics were conducted to evaluate the changes in hepatic lipidomics of broilers. The results showed that there were 12 differential metabolites between the two treatments. Compared with the NT group, HS group reduced the levers of hepatic phosphatidylcholine (PC) (16:0/16:0), PC (16:0/18:2), triglyceride (TG) (16:0/16:1/18:1), TG (18:0/18:1/20:4) (VIP > 1 and p < 0.05), while increased PC (18:1/20:3), PC (18:0/18:1), PC (18:1/18:1), PC (18:0/22:5), dimethyl-phosphatidyl ethanolamine (dMePE) (14:0/18:3), dMePE (18:0/18:1) and dMePE (16:0/20:3) levels (Variable Importance in the Projection; VIP > 1 and p < 0.05). In addition, according to the analysis of metabolic pathway, the pathways of linoleic acid, alpha-linolenic acid, glycerolipid and glycerophospholipid metabolism were involved in the effects of HS on hepatic lipid metabolism of broilers (p < 0.05). In conclusion, HS altered the hepatic lipid metabolism mainly through linoleic acid, alpha-linolenic acid, glycerolipid and glycerophospholipid metabolism pathway in indigenous broilers. These findings provided novel insights into the role of HS on hepatic lipidomics in Chinese indigenous broiler chickens.
高温环境诱导的热应激（HS）是动物的一种危害环境因素，导致其生理和代谢功能发生剧烈变化。然而，慢生长肉鸡肝脏中脂质代谢的代谢组学机制仍不清楚。本研究旨在探讨 HS 对中国本土慢生长肉鸡（淮香鸡）肝脏脂质组学的影响。该研究包括两个处理组，每个处理组有 5 个重复，每个重复有 4 只鸡笼，共有 40 只 8 周龄雌性淮香鸡（平均初始体重为 840.75±20.79g）被随机分为常温（NT）和 HS 组，分别暴露于 21.3±1.2℃和 32.5±1.4℃，两组的相对湿度保持在 55%-70%。采用基于液相色谱-质谱（LC-MS）的代谢组学方法评估肉鸡肝脏脂质组学的变化。结果表明，两组之间有 12 种差异代谢物。与 NT 组相比，HS 组降低了肝脏磷脂酰胆碱（PC）（16:0/16:0）、PC（16:0/18:2）、甘油三酯（TG）（16:0/16:1/18:1）、TG（18:0/18:1/20:4）的水平（VIP>1，p<0.05），而增加了 PC（18:1/20:3）、PC（18:0/18:1）、PC（18:1/18:1）、PC（18:0/22:5）、二甲基-磷酸乙醇胺（dMePE）（14:0/18:3）、dMePE（18:0/18:1）和 dMePE（16:0/20:3）的水平（变量重要性投影；VIP>1，p<0.05）。此外，根据代谢途径分析，亚油酸、α-亚麻酸、甘油酯和甘油磷脂代谢途径参与了 HS 对肉鸡肝脏脂质代谢的影响（p<0.05）。综上所述，HS 通过改变中国本土肉鸡的亚油酸、α-亚麻酸、甘油酯和甘油磷脂代谢途径，改变了肝脏脂质代谢。这些发现为 HS 对中国本土肉鸡肝脏脂质组学的作用提供了新的见解。
