Cai Guangqin, Wang Geliang, Kim Sang-Chul, Li Jianwu, Zhou Yongming, Wang Xuemin
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, Hubei, China.
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Biotechnol Biofuels. 2021 Feb 27;14(1):49. doi: 10.1186/s13068-021-01899-w.
Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied.
Here, we examined the effect of two lipid transporters, FAX1 (fatty acid export1) and ABCA9 (ATP-binding cassette transporter subfamily A9) on oil production and lipid metabolism in the oilseed plant Camelina sativa. Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1-OEs and ABCA9-OEs increasing seed length and width, respectively, whereas FAX1/ABCA9-OEs increasing both. FAX1-OE and ABCA9-OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing pods, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis, such as DGAT2, PDAT1, and LEC1, was increased in developing seeds of FAX1- and/or ABCA9-OEs.
These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelina oil production.
脂质转运蛋白在脂质运输和分布中起重要作用，但它们对油料作物种子油生产的影响尚未得到充分研究。
在此，我们研究了两种脂质转运蛋白，脂肪酸输出蛋白1（FAX1）和ATP结合盒转运蛋白亚家族A9（ABCA9）对油料作物亚麻荠种子油生产和脂质代谢的影响。FAX1和ABCA9的过表达增加了种子重量和大小，其中FAX1过表达株系增加了种子长度，ABCA9过表达株系增加了种子宽度，而FAX1/ABCA9双过表达株系则同时增加了种子长度和宽度。FAX1过表达和ABCA9过表达对种子油含量和种子产量具有累加效应。此外，FAX1和ABCA9的过表达影响了发育中豆荚的膜脂组成，尤其是对磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰甘油的影响。在FAX1和/或ABCA9过表达株系发育中的种子中，一些参与种子油合成的基因，如二酰甘油酰基转移酶2（DGAT2）、磷脂：二酰甘油酰基转移酶1（PDAT1）和胚胎发育晚期丰富蛋白1（LEC1）的表达增加。
这些结果表明，增加FAX1和ABCA9的表达可能有助于提高亚麻荠的油产量。
