Keyzor Charlie, Mermaz Benoit, Trigazis Efstathios, Jo SoYoung, Song Jie
Department of Life Sciences, Imperial College London, London, United Kingdom.
Front Plant Sci. 2021 Feb 12;12:640135. doi: 10.3389/fpls.2021.640135. eCollection 2021.
The chromatin modification H3K27me3 is involved in almost every developmental stage in Arabidopsis. Much remains unknown about the dynamic regulation of this histone modification in flower development and control of self-fertility. Here we demonstrate that the H3K27me3-specific demethylases ELF6 and JMJ13 antagonistically regulate carpel and stamen growth and thus modulate self-fertility. Transcriptome and epigenome data are used to identify potential targets of ELF6 and JMJ13 responsible for these physiological functions. We find that ELF6 relieves expansin genes of epigenetic silencing to promote cell elongation in the carpel, enhancing carpel growth and therefore encouraging out-crossing. On the other hand, JMJ13 activates genes of the jasmonic acid regulatory network alongside the auxin responsive SAUR26, to inhibit carpel growth, enhance stamen growth, and overall promote self-pollination. Our evidence provides novel mechanisms of self-fertility regulation in demonstrating how chromatin modifying enzymes govern the equilibrium between flower self-pollination and out-crossing.
染色质修饰H3K27me3参与拟南芥几乎每个发育阶段。关于这种组蛋白修饰在花发育和自育性控制中的动态调控，仍有许多未知之处。在这里，我们证明H3K27me3特异性去甲基化酶ELF6和JMJ13拮抗调节心皮和雄蕊生长，从而调节自育性。转录组和表观基因组数据用于确定负责这些生理功能的ELF6和JMJ13的潜在靶标。我们发现ELF6解除了扩展蛋白基因的表观遗传沉默，以促进心皮中的细胞伸长，增强心皮生长，从而促进异交。另一方面，JMJ13与生长素响应性SAUR26一起激活茉莉酸调节网络的基因，以抑制心皮生长，增强雄蕊生长，并总体上促进自花授粉。我们的证据提供了自育性调控的新机制，证明了染色质修饰酶如何控制花自花授粉和异交之间的平衡。
