Department of Endodontics, School of Dentistry, State University of Rio de Janeiro (UERJ), Rio de Janeiro, RJ, Brazil.
Department of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, Brazil.
Dent Mater. 2021 May;37(5):863-874. doi: 10.1016/j.dental.2021.02.007. Epub 2021 Feb 27.
The aim of this study was to evaluate the influence of the incorporation of chlorhexidine-hexametaphosphate nanoparticles (CHX-HMP NPs) on antibacterial, cytotoxic and physicochemical properties of AH Plus (AH), MTA Fillapex (MTA) and Pulp Canal Sealer (PCS).
The NPs were synthesized and characterized by Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), zeta potential, Atomic Force Microscopy (AFM) and Energy-Dispersive X-ray Spectroscopy (EDS). The incorporation was made by weight, 2% and 5% of NPs. The antimicrobial activity, cytotoxicity, flow, radiopacity, setting time, solubility and pH were evaluated. The statistical analysis was performed by two-way analysis of variance test and Tukey post hoc test (P < 0.05).
SEM analysis showed the tendency for CHX-HMP NPs to cluster, the effective mean diameter measured by DLS: 169.39 nm and the zeta potential: -10.18 mV. The NPs were individually measured by AFM: 22.99-52.75 nm. EDS analysis identified the presence of C, N, O, Na, P, Cl. After incorporation: The Direct Contact Test showed an increase in the antimicrobial action of AH, PCS and MTA; the sealers showed a decrease in flow and at 24 h of immersion also an increase in solubility, but did not affect the radiopacity of the samples; AH setting time increased and MTA did not reach setting under any of the conditions tested. All samples showed a decrease in pH value as the immersion time progressed.
The incorporation of NPs can improve the antimicrobial performance of endodontic sealers without impairing other biological and physicochemical properties.
本研究旨在评估氯己定-六亚甲基膦酸纳米颗粒（CHX-HMP NPs）的掺入对 AH Plus（AH）、MTA Fillapex（MTA）和牙髓腔封闭剂（PCS）的抗菌、细胞毒性和理化性能的影响。
通过扫描电子显微镜（SEM）、动态光散射（DLS）、zeta 电位、原子力显微镜（AFM）和能谱分析（EDS）对 NPs 进行合成和表征。通过重量、2%和 5%的 NPs 进行掺入。评估了抗菌活性、细胞毒性、流动度、射线不透性、凝固时间、溶解度和 pH 值。采用双向方差分析和 Tukey 事后检验（P < 0.05）进行统计分析。
SEM 分析显示 CHX-HMP NPs 有团聚的趋势，DLS 测量的有效平均粒径为 169.39nm，zeta 电位为-10.18mV。NPs 经 AFM 单独测量为 22.99-52.75nm。EDS 分析确定存在 C、N、O、Na、P、Cl。掺入后：直接接触试验显示 AH、PCS 和 MTA 的抗菌作用增强；密封剂的流动度降低，在 24 小时浸泡时也增加了溶解度，但不影响样品的射线不透性；AH 的凝固时间增加，而在任何测试条件下 MTA 都没有凝固。所有样品的 pH 值随浸泡时间的延长而降低。
NPs 的掺入可以提高根管封闭剂的抗菌性能，而不损害其他生物学和理化性能。
