Centro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660, Boadilla del Monte, Spain.
Laboratorio Cajal de Circuitos Corticales (CTB), Universidad Politécnica de Madrid, Madrid, Spain.
Mol Neurobiol. 2021 Jul;58(7):3224-3237. doi: 10.1007/s12035-020-02264-y. Epub 2021 Mar 2.
Brain slice preparations are widely used for research in neuroscience. However, a high-quality preparation is essential and there is no consensus regarding stable parameters that can be used to define the status of the brain slice preparation after its collection at different time points. Thus, it is critical to fully characterize the experimental conditions for ex vivo studies using brain slices for electrophysiological recording. In this study, we used a multiplatform (LC-MS and GC-MS) untargeted metabolomics-based approach to shed light on the metabolome and lipidome changes taking place at different time intervals during the brain slice preparation process. We have found significant modifications in the levels of 300 compounds, including several lipid classes and their derivatives, as well as metabolites involved in the GABAergic pathway and the TCA cycle. All these preparation-dependent changes in the brain biochemistry related to the time interval should be taken into consideration for future studies to facilitate non-biased interpretations of the experimental results.
脑片制备广泛应用于神经科学研究。然而，高质量的制备是必不可少的，对于在不同时间点收集的脑片制备物，没有关于可用于定义其状态的稳定参数的共识。因此，对于使用脑片进行电生理记录的离体研究，充分描述实验条件至关重要。在这项研究中，我们使用了一种多平台（LC-MS 和 GC-MS）非靶向代谢组学方法，揭示了脑片制备过程中不同时间间隔内代谢组和脂质组发生的变化。我们发现了 300 种化合物水平的显著变化，包括几种脂质类及其衍生物，以及参与 GABA 能途径和 TCA 循环的代谢物。与时间间隔相关的与脑生化有关的所有这些制备依赖性变化都应在未来的研究中加以考虑，以便对实验结果进行无偏解释。
