Medina M A, Sánchez-Jiménez F, Márquez F J, Pérez-Rodríguez J, Quesada A R, Núñez de Castro I
Departamento de Bioquímica, Biología Molecular y Química Orgánica, Facultad de Ciencias, Universidad de Málaga, Spain.
Biochem Int. 1988 Feb;16(2):339-47.
Energy metabolism of freshly harvested Ehrlich ascites tumour cells in the presence of 5 mM glucose and/or 0.5 mM glutamine was studied. The rate of oxygen utilization was not altered by the addition of 0.5 mM glutamine; 5 mM glucose induced an inhibition of respiration. In the presence of both glucose and glutamine, the Crabtree effect decreased. In these conditions, the rates of oxygen uptake, the CO2 evolution and the changes in the redox states of cytochromes indicate that glucose is preferred by Ehrlich ascites tumour cells as energy substrate. Glucose decreased the rate of glutamine utilization by 34%. On the other hand, glutaminolysis did not inhibit glycolysis.
研究了在5 mM葡萄糖和/或0.5 mM谷氨酰胺存在下新鲜收获的艾氏腹水癌细胞的能量代谢。添加0.5 mM谷氨酰胺不会改变氧气利用率；5 mM葡萄糖会抑制呼吸作用。在葡萄糖和谷氨酰胺同时存在的情况下，克氏效应减弱。在这些条件下，氧气摄取速率、二氧化碳释放速率以及细胞色素氧化还原状态的变化表明，艾氏腹水癌细胞更倾向于将葡萄糖作为能量底物。葡萄糖使谷氨酰胺利用率降低了34%。另一方面，谷氨酰胺分解并未抑制糖酵解。
