Lefford M J, Mackaness G B
Infect Immun. 1977 Nov;18(2):363-9. doi: 10.1128/iai.18.2.363-369.1977.
After injection of 10(8) live Mycobacterium lepraemurium (MLM) into the left hind footpad of mice, there is development of local swelling attributable to a granuloma of the cell-mediated immunity type. Concomitant intravenous inoculation of live MLM delays and may even suppress footpad swelling, the effects being proportional to the intravenous dose of organisms. Concomitant footpad infection and intravenous inoculation of 10(9) dead MLM also delays footpad swelling, but over a period of months the feet become excessively swollen. The excessive swelling is due to local enhancement of infection as evidenced by an increase in the number of MLM per footpad. Attempts were made to prevent such immunosuppression by splenectomy or treatment with BCG. Splenectomy was entirely without effect, but 10(7) live BCG administered intravenously 2 to 4 weeks before dead MLM prevented enhancement of infection. The mediator of the immunosuppressive mechanism that results in enhanced infection remains to be elucidated, but it is unlikely to be antibody or immune complexes.
给小鼠左后足垫注射10⁸ 活菌型鼠麻风杆菌（MLM）后，会出现因细胞介导免疫型肉芽肿引起的局部肿胀。同时静脉接种活菌型MLM会延迟甚至可能抑制足垫肿胀，其效果与静脉注射的菌量成正比。同时进行足垫感染和静脉接种10⁹ 死菌型MLM也会延迟足垫肿胀，但数月后足部会过度肿胀。过度肿胀是由于感染的局部增强，这可通过每个足垫中MLM数量的增加得到证明。曾尝试通过脾切除术或卡介苗（BCG）治疗来预防这种免疫抑制。脾切除术完全无效，但在死菌型MLM接种前2至4周静脉注射10⁷ 活菌型BCG可防止感染增强。导致感染增强的免疫抑制机制的介质仍有待阐明，但不太可能是抗体或免疫复合物。
