Lefford M J, Morgan R, Logie P S
Infect Immun. 1980 Jun;28(3):860-6. doi: 10.1128/iai.28.3.860-866.1980.
Mice were infected with 10(8) Mycobacterium lepraemurium in the footpad (unsuppressed mice), and some of these animals were concurrently given 10(9) heat-killed M. lepraemurium intravenously (suppressed mice). These groups of mice were preimmunized with 10(7) viable organisms of Mycobacterium bovis BCG by several routes. BCG inhibited the proliferation of M. lepraemurium in the unsuppressed mice, but not in the suppressed mice. In effect, the intravenous administration of heat-killed M. lepraemurium suppressed the immunity to M. lepraemurium that BCG vaccination had engendered. BCG did not protect normal mice against intravenous infection with M. lepraemurium. It appears that normal mice against intravenous infection with M. lepraemurium. It appears that the inhibitory effect of BCG vaccination upon M. lepraemurium infection is due to cross-reactive immunity rather than to nonspecific immunity or immunopotentiation. Thus, the route of BCG vaccination was immaterial, and vaccination 12 weeks before M. lepraemurium infection was as beneficial as vaccination 4 weeks before infection. Moreover, spleen cells from M. lepraemurium-immunized mice conferred adoptive immunity to BCG. The implications of this study for the use of BCG as a prophylactic and therapeutic agent in human leprosy are discussed.
将10⁸ 鼠麻风杆菌接种于小鼠足垫（未受抑制的小鼠），其中一些动物同时静脉注射10⁹ 热灭活的鼠麻风杆菌（受抑制的小鼠）。这些小鼠组通过多种途径用10⁷ 活的牛型结核分枝杆菌卡介苗进行预免疫。卡介苗抑制了未受抑制小鼠体内鼠麻风杆菌的增殖，但对受抑制的小鼠没有作用。实际上，静脉注射热灭活的鼠麻风杆菌抑制了卡介苗接种所产生的对鼠麻风杆菌的免疫力。卡介苗不能保护正常小鼠免受静脉注射鼠麻风杆菌的感染。看来正常小鼠对静脉注射鼠麻风杆菌有抵抗力。卡介苗接种对鼠麻风杆菌感染的抑制作用似乎是由于交叉反应性免疫，而不是非特异性免疫或免疫增强作用。因此，卡介苗接种途径并不重要，在感染鼠麻风杆菌前12周接种与在感染前4周接种一样有益。此外，来自鼠麻风杆菌免疫小鼠的脾细胞赋予了对卡介苗的过继免疫。本文讨论了该研究对卡介苗作为人类麻风病预防和治疗剂使用的意义。
