Qin Chong, Zhang Xiaowei, Cui Zongqiang
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, P. R. China.
University of Chinese Academy of Sciences, 100049 Beijing, P. R. China.
Bio Protoc. 2019 Sep 20;9(18):e3366. doi: 10.21769/BioProtoc.3366.
The genome of influenza A virus (IAV) comprises eight pinlike genomic segments called vRNPs enclosed in viral capsid. During infection, uncoating is the key step for viral replication and represents an antiviral therapeutic target, but it is difficult to observe the transient and dynamic event in detail. Here, we report a protocol for production of quantum dots-containing influenza virus particles by encapsulating quantum dot-conjugated vRNPs during viral assembly. These labeled virions can be used for monitoring viral trafficking in real time and studying viral uncoating processes.
甲型流感病毒（IAV）的基因组由八个针状基因组片段组成，这些片段称为vRNPs，包裹在病毒衣壳中。在感染过程中，脱壳是病毒复制的关键步骤，也是抗病毒治疗的靶点，但很难详细观察到这一短暂而动态的过程。在此，我们报告了一种在病毒组装过程中通过封装量子点共轭的vRNPs来生产含量子点的流感病毒颗粒的方法。这些标记的病毒粒子可用于实时监测病毒运输和研究病毒脱壳过程。
