College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China.
Center for Advanced Analytical Science, School of Chemistry and Chemical Engineering c/o School of Civil Engineering, Guangzhou University, Guangzhou, 510006, China.
Angew Chem Int Ed Engl. 2021 May 17;60(21):11774-11778. doi: 10.1002/anie.202101468. Epub 2021 Apr 16.
Microelectrode-based electrochemical (EC) and photoelectrochemical (PEC) sensors are promising candidates for in vivo analysis of biologically important chemicals. However, limited selectivity in complicated biological systems and poor adaptability to electrochemically non-active species restrained their applications. Herein, we propose the concept of modulating the PEC output by a fluorescence resonance energy transfer (FRET) process. The emission of energy donor was dependent on the concentration of target SO , which in turn served as the modulator of the photocurrent signal of the photoactive material. The employment of optical modulation circumvented the problem of selectivity, and the as-fabricated PEC microelectrode showed good stability and reproducibility in vivo. It can monitor fluctuations of SO levels in brains of rat models of cerebral ischemia-reperfusion and febrile seizure. More significantly, such a FRET modulated signaling strategy can be extended to diverse analytes.
基于微电极的电化学（EC）和光电化学（PEC）传感器是用于体内分析生物重要化学物质的有前途的候选者。然而，在复杂的生物系统中选择性有限以及对电化学非活性物质的适应性差限制了它们的应用。在此，我们提出了通过荧光共振能量转移（FRET）过程来调节 PEC 输出的概念。能量供体的发射取决于目标 SO 的浓度，而 SO 又反过来充当光活性材料光电流信号的调节剂。采用光学调制避免了选择性问题，所制备的 PEC 微电极在体内显示出良好的稳定性和重现性。它可以监测脑缺血再灌注和热性惊厥大鼠模型中 SO 水平的波动。更重要的是，这种 FRET 调制信号策略可以扩展到多种分析物。
