Wang Xiaoying, Wei Wei, Zhao Jing
State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China.
School of Life Sciences, Nanjing University, Nanjing, China.
Front Chem. 2021 Feb 15;9:631909. doi: 10.3389/fchem.2021.631909. eCollection 2021.
Intracellular concentrations of essential mental ions must be tightly maintained to avoid metal deprivation and toxicity. However, their levels in cells are still difficult to monitor. In this report, the combination of a CoNi-specific riboswitch and an engineered downstream mCherry fluorescent protein allowed a highly sensitive and selective whole-cell Co/Ni detection process. The sensors were applied to examine the resistance system of Co/Ni , and the sensors were able to monitor the effects of genetic deletions. These results indicate that riboswitch-based sensors can be employed in the study of related cellular processes.
必需金属离子的细胞内浓度必须严格维持，以避免金属缺乏和毒性。然而，它们在细胞中的水平仍然难以监测。在本报告中，钴镍特异性核糖开关与工程化的下游mCherry荧光蛋白相结合，实现了高度灵敏和选择性的全细胞钴/镍检测过程。这些传感器被用于检测钴/镍的抗性系统，并且能够监测基因缺失的影响。这些结果表明，基于核糖开关的传感器可用于相关细胞过程的研究。
