Garcia-Gomes Mariana S A, Zanatto Dennis A, Yamamoto Pedro K, Wadt Danilo, Antiorio Ana T F B, Aleman-Laporte Jilma, Alexandre-Ribeiro Sandra R, Marson Guilherme A, Guizzo Cezar, Massironi Silvia M G, Bernardi Maria M, Mori Claudia M C
Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo (USP), São Paulo, Brazil.
Department of Immunology, Institute of Biomedical Science, University of São Paulo (USP), São Paulo, Brazil.
Bio Protoc. 2020 Apr 5;10(7):e3568. doi: 10.21769/BioProtoc.3568.
Despite the great number of test batteries already known to assess the behavior of genetically modified and inbred strains of mice, only a few of them focus on basic neurological parameters. The purpose of the battery test proposed is to settle a specific methodology to characterize the phenotype of neurological disease models in mutant or genetically modified mice. This methodology is simple and efficient in order to analyze several parameters, including general activity, sensory nervous system, sensorimotor system, central nervous system and autonomous nervous system. This can aid the choice of specific additional tests as well as the determination of an interrelationship among phenotypic alterations observed. Although being efficient for a first analysis of a mouse model, interpretation of the results must be carefully made because phenotype manifestation may vary due to many parameters, including mouse strain, environmental and housing condition, animal-experimenter interaction, sample size and tests order. It is important to consider as a critical point if handling procedures are aversive. The results acquired with the analysis of 18 parameters together provide preliminary data to characterize mouse phenotype and helps selecting more specific tests.
尽管已知有大量的测试组合可用于评估基因改造和近交系小鼠的行为，但其中只有少数关注基本神经学参数。所提出的组合测试的目的是确定一种特定方法，以表征突变或基因改造小鼠中神经疾病模型的表型。这种方法简单有效，可用于分析多个参数，包括一般活动、感觉神经系统、感觉运动系统、中枢神经系统和自主神经系统。这有助于选择特定的附加测试，以及确定所观察到的表型改变之间的相互关系。尽管该方法对小鼠模型的初步分析很有效，但由于许多参数（包括小鼠品系、环境和饲养条件、动物与实验者的相互作用、样本大小和测试顺序）的影响，表型表现可能会有所不同，因此在解读结果时必须谨慎。如果处理程序令人厌恶，将其视为一个关键点很重要。对18个参数进行分析所获得的结果共同提供了表征小鼠表型的初步数据，并有助于选择更具体的测试。
