College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang, People's Republic of China.
Key Laboratory of Plants Genetic Engineering Center, Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, Hebei, People's Republic of China.
Curr Microbiol. 2021 Apr;78(4):1678-1685. doi: 10.1007/s00284-021-02403-1. Epub 2021 Mar 5.
A Gram-negative-staining, endospore-forming, rod-shaped bacterium, designated T1, was isolated from root nodules of soybean (Glycine max (L.) Merr) in Heilongjiang Province of China. The isolate was identified as a member of the genus Paenibacillus based on phenotypic and phylogenetic characteristics. The 16S rRNA sequence was closely related to that of Paenibacillus sacheonensis SY01 with a similarity of 98.4%. Average nucleotide identity and in silico DNA-DNA hybridization values between strain T1 and P. sacheonensis DSM 23054 were 81.4% and 25.4%, respectively. The DNA G + C content of strain T1 was 58.2 mol%. meso-Diaminopimelic acid was detected in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C, iso-C and iso-C. The predominant respiratory quinone was MK-7. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified phospholipids, four unidentified aminophospholipids, an unidentified glycolipid and an unidentified lipid. Based on these results, T1 is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus glycinis sp. nov. is proposed. The type strain is T1 (= CGMCC 1.18563 = KCTC43227).
一株革兰氏阴性、产芽孢、杆状细菌，命名为 T1，从中国黑龙江省的大豆（Glycine max (L.) Merr）根瘤中分离得到。该分离株基于表型和系统发育特征被鉴定为类芽孢杆菌属的一个成员。16S rRNA 序列与 Paenibacillus sacheonensis SY01 最为相似，相似度为 98.4%。T1 株与 Paenibacillus sacheonensis DSM 23054 的平均核苷酸同一性和 DNA-DNA 杂交值分别为 81.4%和 25.4%。T1 株的 DNA G+C 含量为 58.2 mol%。细胞壁肽聚糖中检测到 meso-二氨基庚二酸。主要细胞脂肪酸为 anteiso-C、iso-C 和 iso-C。主要呼吸醌为 MK-7。极性脂类为二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、五种未鉴定的磷脂、四种未鉴定的氨基磷脂、一种未鉴定的糖脂和一种未鉴定的脂类。基于这些结果，T1 被认为代表了类芽孢杆菌属的一个新种，因此提议将其命名为 Glycine max 解淀粉芽孢杆菌（Paenibacillus glycinis）sp. nov.。模式菌株为 T1（= CGMCC 1.18563 = KCTC43227）。
