Wang Yung-Chih, Tsai Yao-Hung, Shen Ching-Fen, He Ming-Yao, Fu Yi-Chen, Sang Chen-Yu, Lee Yi-Tzu, Cheng Chao-Min
National Defense Medical Center, Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, Taipei 114, Taiwan.
Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.
Micromachines (Basel). 2021 Feb 13;12(2):194. doi: 10.3390/mi12020194.
has been known to cause a variety of infectious diseases. The conventional enzyme-linked immunosorbent assay (ELISA) is a well-known method widely used to diagnose a variety of infectious diseases. This method is expensive and requires considerable time and effort to conduct and complete multiple integral steps. We previously proposed the use of paper-based ELISA to rapidly detect the presence of . This approach has demonstrated utility for point-of-care (POC) urinary tract infection diagnoses. Paper-based ELISA, while advantageous, still requires the execution of several procedural steps. Here, we discuss the design and experimental implementation of a turntable paper-based device to simplify the paper-based ELISA protocols for the detection of . In this process, antibodies or reagents are preloaded onto zones of a paper-based device and allowed to dry before use. We successfully used this device to detect with a detection limit of 10 colony-forming units (colony-forming unit [CFU])/mL.
已知会引发多种传染病。传统的酶联免疫吸附测定法（ELISA）是一种广为人知的方法，被广泛用于诊断多种传染病。该方法成本高昂，且需要大量时间和精力来执行并完成多个完整步骤。我们之前曾提议使用基于纸的ELISA来快速检测……的存在。这种方法已证明可用于即时护理（POC）尿路感染诊断。基于纸的ELISA虽有优势，但仍需要执行多个程序步骤。在此，我们讨论一种基于转盘的纸质设备的设计与实验实施，以简化用于检测……的基于纸的ELISA方案。在此过程中，抗体或试剂预先加载到基于纸的设备区域上，并在使用前晾干。我们成功使用该设备检测……，检测限为10个菌落形成单位（CFU）/毫升。
