Thaller M C, Berlutti F, Dainelli B, Pezzi R
Instituto di Microbiologia, Università, La Sapienza, Rome, Italy.
J Clin Microbiol. 1988 Apr;26(4):791-3. doi: 10.1128/jcm.26.4.791-793.1988.
A new selective, differential plating medium to screen the common gram-negative urinary tract pathogens is described. The medium combines adonitol fermentation, phenylalanine deaminase, and beta-glucuronidase tests and allows the indole and cytochrome oxidase tests to be performed directly from the plates. High-level agreement with individual conventional tests was recorded in comparative studies with 504 cultures of gram-negative rods. There was 100% agreement, except for the Providencia spp. indole spot test (61.6% agreement). Adonitol fermentation by Providencia species could not be determined. Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa were identified with a high efficiency (100, 85.7, 83.5, and 100% agreement, respectively) without further testing. There was 96% overall agreement for the 267 infected urine samples tested.
本文描述了一种用于筛选常见革兰氏阴性尿路病原体的新型选择性、鉴别性平板培养基。该培养基结合了阿东糖醇发酵、苯丙氨酸脱氨酶和β-葡萄糖醛酸酶试验，并允许直接在平板上进行吲哚和细胞色素氧化酶试验。在对504株革兰氏阴性杆菌培养物的比较研究中，与各个传统试验的一致性很高。除普罗威登斯菌属吲哚斑点试验（一致性为61.6%）外，一致性为100%。无法确定普罗威登斯菌属对阿东糖醇的发酵情况。无需进一步检测，即可高效鉴定大肠杆菌、肺炎克雷伯菌、奇异变形杆菌和铜绿假单胞菌（一致性分别为100%、85.7%、83.5%和100%）。对267份受感染尿液样本进行检测，总体一致性为96%。
