Berlutti F, Thaller M C, Dainelli B, Pezzi R
Istituto di Microbiologia, Università La Sapienza, Rome, Italy.
J Clin Microbiol. 1989 Jul;27(7):1646-9. doi: 10.1128/jcm.27.7.1646-1649.1989.
In this paper, we describe a reduced sequence of identification that includes T-mod medium, a selective and differential isolation medium which allows accurate presumptive identification of the most common gram-negative bacteria encountered in urine samples. The present study, performed on bacteria isolated from 1,762 independent urine samples, has shown that a few selected tests (lysine and ornithine decarboxylase, urease and trehalose fermentation tests) improve the identification accuracy of T-mod, making it possible both to identify the less frequent species and to prevent some misidentifications of Klebsiella pneumoniae and Proteus mirabilis. The proposed work flow agreed with conventional identification protocols to a 99.3% extent and allowed identification of 87.4% of the isolates directly from the primary plate, 11.4% after 1 to 3 additional tests, and 1.2% after an identification gallery.
在本文中，我们描述了一种简化的鉴定流程，其中包括T-mod培养基，这是一种选择性和鉴别性分离培养基，可对尿液样本中最常见的革兰氏阴性菌进行准确的初步鉴定。本研究对从1762份独立尿液样本中分离出的细菌进行了检测，结果表明，一些选定的试验（赖氨酸和鸟氨酸脱羧酶、脲酶和海藻糖发酵试验）提高了T-mod的鉴定准确性，既能鉴定出较少见的菌种，又能防止肺炎克雷伯菌和奇异变形杆菌的一些错误鉴定。所提出的工作流程与传统鉴定方案的符合程度达到99.3%，能够直接从原始平板上鉴定出87.4%的分离株，在进行1至3次额外试验后鉴定出11.4%的分离株，在使用鉴定菌谱后鉴定出1.2%的分离株。
