Selan L, Berlutti F, Passariello C, Thaller M C, Renzini G
Istituto di Microbiologia, Facoltà di Farmacia, Università degli Studi La Sapienza, Rome, Italy.
J Clin Microbiol. 1992 Jul;30(7):1739-42. doi: 10.1128/jcm.30.7.1739-1742.1992.
The reliability of bioluminescence assays which employ the luciferin-luciferase ATP-dependent reaction to evaluate bacterial counts was studied, both in vitro and on urine specimens. Bioluminescence and cultural results for the most common urinary tract pathogens were analyzed. Furthermore, the influence of the culture medium, of the assaying method, and of the phase of growth on bioluminescence readings was studied. Results show that Proteus, Providencia, and Morganella strains are not correctly detected, neither in vitro nor in urine samples, by the standard assaying method. The analysis of assaying parameters demonstrated that some modifications to the extraction procedure of bacterial ATP could improve the reliability of this technique.
研究了利用荧光素-荧光素酶ATP依赖性反应评估细菌数量的生物发光测定法在体外和尿液样本中的可靠性。分析了最常见尿路病原体的生物发光和培养结果。此外，还研究了培养基、测定方法和生长阶段对生物发光读数的影响。结果表明，标准测定方法在体外和尿液样本中均不能正确检测变形杆菌、普罗威登斯菌和摩根菌菌株。对测定参数的分析表明，对细菌ATP提取程序进行一些修改可以提高该技术的可靠性。
