评估酶联免疫吸附测定（ELISA）、反向被动乳胶凝集试验（RPLA）和Vero细胞检测法在粪便标本中检测产气荚膜梭菌肠毒素的效果。
Evaluation of ELISA, RPLA, and Vero cell assays for detecting Clostridium perfringens enterotoxin in faecal specimens.
作者信息
Berry P R, Rodhouse J C, Hughes S, Bartholomew B A, Gilbert R J
机构信息
Food Hygiene Laboratory, Central Public Health Laboratory, London.
出版信息
J Clin Pathol. 1988 Apr;41(4):458-61. doi: 10.1136/jcp.41.4.458.
Abstract
Three hundred and ninety two faecal specimens from 70 separate outbreaks of suspected Clostridium perfringens food poisoning were examined by enzyme linked immunosorbent assay (ELISA), reversed passive latex agglutination (RPLA), and Vero cell assays for the presence of enterotoxin. Although the most time consuming method, ELISA was the most specific and reproducible. RPLA was slightly more sensitive than ELISA, but it showed some non-specific reactions. The Vero cell assay was the least sensitive and least reproducible method, being affected by some non-specific cytotoxic and cytotonic reactions. Normal rabbit serum should be included in the Vero cell assay as a control for the neutralisation of cytotoxic effects.
摘要
采用酶联免疫吸附测定（ELISA）、反向被动乳胶凝集试验（RPLA）和Vero细胞试验，对来自70起疑似产气荚膜梭菌食物中毒独立暴发事件的392份粪便标本进行了肠毒素检测。尽管ELISA是最耗时的方法，但它却是最具特异性和可重复性的。RPLA比ELISA稍敏感一些，但它显示出一些非特异性反应。Vero细胞试验是最不敏感且最不可重复的方法，受到一些非特异性细胞毒性和细胞兴奋性反应的影响。Vero细胞试验中应加入正常兔血清作为中和细胞毒性作用的对照。
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