Muhammad Hussin, Omar Maizatul Hasyima, Rasid Elda Nurafnie Ibnu, Suhaimi Shazlan Noor, Mohkiar Farah Huda, Siu Lau Mei, Awang Norizah
Herbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Ministry of Health, Malaysia, Level 5, Block C7, No. 1, Jalan Setia Murni U13/52, Seksyen U13, Setia Alam, Shah Alam 40170, Selangor, Malaysia.
Plants (Basel). 2021 Feb 11;10(2):343. doi: 10.3390/plants10020343.
The present study was carried out to assess the genotoxicity potential of var. aqueous extract (FDAE) using standard in vitro assays. The DNA damage of V79B cells was measured using the alkaline comet assay treated at 0.1 mg/mL (IC10) and 0.3 mg/mL (IC25) of FDAE together with positive and negative controls. For in vitro micronucleus assay, the V79B cells were treated with FDAE at five different concentrations (5, 2.5, 1.25, 0.625, and 0.3125 mg/mL) with and without S9 mixture. The bacteria reverse mutation assay of FDAE was performed on strains TA98, 100, 1535, 1537, and strain WP2 using pre-incubation method in the presence or in the absence of an extrinsic metabolic system (S9 mixture). FDAE at 0.1 and 0.3 mg/mL significantly increased DNA damage in both comet tail and tail moment ( < 0.05). No significant changes were detected in the number of micronucleated cell when compared to control. Tested at the doses up to 5000 µg/plate, the FDAE did not increase the number of revertant colonies for all strains. In conclusion, further investigation needs to be conducted in animal model to confirm the non-genotoxicity activities of FDAE.
本研究旨在使用标准体外试验评估变种水提取物（FDAE）的潜在遗传毒性。使用碱性彗星试验测量V79B细胞的DNA损伤，将细胞用0.1 mg/mL（IC10）和0.3 mg/mL（IC25）的FDAE处理，同时设置阳性和阴性对照。对于体外微核试验，将V79B细胞用FDAE的五种不同浓度（5、2.5、1.25、0.625和0.3125 mg/mL）处理，有无S9混合物。使用预孵育方法，在有或无外源代谢系统（S9混合物）的情况下，对FDAE进行TA98、100、1535、1537菌株和WP2菌株的细菌回复突变试验。0.1和0.3 mg/mL的FDAE显著增加了彗星尾和尾矩中的DNA损伤（<0.05）。与对照相比，微核细胞数量未检测到显著变化。在高达5000 µg/平板的剂量下进行测试，FDAE对所有菌株均未增加回复菌落数。总之，需要在动物模型中进行进一步研究以确认FDAE的非遗传毒性活性。
