Faculty of Chemistry, University of Wroclaw, Joliot-Curie 14, 50-383 Wroclaw, Poland.
Department of Biotransformation, Faculty of Biotechnology, University of Wroclaw, Joliot-Curie 14, 50-383 Wroclaw, Poland.
Molecules. 2021 Feb 18;26(4):1063. doi: 10.3390/molecules26041063.
The skin is constantly exposed to external and internal factors that disturb its function. In this work, two nanosystems-levan nanoparticles and a surfactin-stabilized nanoemulsion were preserved (tested for microbial growth) and characterized (size, polydispersity, Zeta potential, and stability). The nanosystems were introduced in the model formulations-cream, tonic, and gel, and confirmed by TEM. The analysis showed that nanoemulsion has a spherical morphology and size 220-300 nm, while levan nanoparticles had irregular shapes independently of the use of matrix and with particle size (130-260 nm). Additionally, we examined the antiradical effect of levan nanoparticles and nanoemulsion in the prototype of formulations by scavenging DPPH (2,2-diphenyl-1-picrylhydrazyl; EPR spectroscopy). The model cream with both nanosystems and the whole range of products with nanosystems were evaluated in vivo for hydration, elasticity, smoothness, wrinkles and vascular lesions, discoloration, respectively. The cream improved skin condition in all tested parameters in at least 50% of volunteers. The use of more comprehensive care, additionally consisting of a tonic and gel, reduced the previously existing skin discoloration to 10.42 ± 0.58%. The presented prototype formulations are promising in improving skin conditions.
皮肤不断受到内外因素的干扰，这些因素会破坏其功能。在这项工作中，保存了两种纳米系统-纤维二糖纳米颗粒和表面活性剂稳定的纳米乳液（测试微生物生长）并进行了表征（大小、多分散性、Zeta 电位和稳定性）。纳米系统被引入模型配方-霜剂、爽肤水和凝胶中，并通过 TEM 进行了确认。分析表明，纳米乳液具有球形形态和 220-300nm 的粒径，而纤维二糖纳米颗粒则具有不规则形状，这与基质的使用无关，且粒径为（130-260nm）。此外，我们通过 DPPH（2,2-二苯基-1-苦基肼；EPR 光谱）在制剂原型中检查了纤维二糖纳米颗粒和纳米乳液的抗氧化作用。含有两种纳米系统的模型霜剂以及含有纳米系统的整个系列产品均在体内评估了其保湿、弹性、光滑度、皱纹和血管损伤、变色等方面的效果。在至少 50%的志愿者中，该霜剂改善了所有测试参数的皮肤状况。使用更全面的护理，包括爽肤水和凝胶，可将先前存在的皮肤变色减少到 10.42±0.58%。所提出的原型配方有望改善皮肤状况。
