Medical Technology Research Centre, Anglia Ruskin University, Chelmsford CM1 1SQ, UK.
RM Consulting, San Diego, CA 92037, USA.
Int J Mol Sci. 2021 Feb 28;22(5):2459. doi: 10.3390/ijms22052459.
Although molecular testing, and RT-qPCR in particular, has been an indispensable component in the scientific armoury targeting SARS-CoV-2, there are numerous falsehoods, misconceptions, assumptions and exaggerated expectations with regards to capability, performance and usefulness of the technology. It is essential that the true strengths and limitations, although publicised for at least twenty years, are restated in the context of the current COVID-19 epidemic. The main objective of this commentary is to address and help stop the unfounded and debilitating speculation surrounding its use.
尽管分子检测，尤其是 RT-qPCR，已成为针对 SARS-CoV-2 的科学武器库中不可或缺的组成部分，但该技术的能力、性能和实用性方面存在着众多的虚假、误解、假设和夸大的期望。重要的是，尽管该技术的真正优势和局限性已经宣传了至少二十年，但在当前 COVID-19 疫情的背景下需要重申这些内容。本评论的主要目的是解决并帮助阻止围绕其使用的毫无根据和削弱信心的猜测。
