Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRA USC1328, Université d'Orléans, Eure et Loir Campus, 21 rue de Loigny la Bataille, F-28000 Chartres, France.
Biomolecules. 2021 Feb 17;11(2):299. doi: 10.3390/biom11020299.
L. is traditionally used as antibacterial medicine and accumulates many antioxidant phytochemicals. Here, we expand this traditional usage with the green biosynthesis of silver nanoparticles (AgNPs) achieved using a L. leaf extract as a reducing and capping agent. The green synthesis of AgNPs reaction was carried out using 1mM silver nitrate and leaf extract. The effect of temperature on the synthesis of AgNPs was examined using room temperature (25 °C) and 60 °C. The silver nanoparticles were formed in one hour by stirring at room temperature. In this case, a yellowish brown colour was developed. The successful formation of silver nanoparticles was confirmed by UV-Vis, Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) analysis. The characteristic peaks of the UV-vis spectrum and XRD confirmed the synthesis of AgNPs. The biosynthesised AgNPs showed potential antioxidant activity through DPPH assay. These AgNPs also exhibited potential antibacterial activity against human pathogenic bacteria. The results were compared with the antioxidant and antibacterial activities of the plant extract, and clearly suggest that the green biosynthesized AgNPs can constitute an effective antioxidant and antibacterial agent.
L. 传统上被用作抗菌药物，并积累了许多抗氧化植物化学物质。在这里，我们通过使用 L. 叶提取物作为还原剂和封端剂来实现银纳米粒子 (AgNPs) 的绿色生物合成，扩展了这种传统用途。AgNPs 的绿色合成反应使用 1mM 硝酸银和叶提取物进行。使用室温（25°C）和 60°C 检查了温度对 AgNPs 合成的影响。在室温下搅拌一小时即可形成银纳米粒子。在这种情况下，会产生黄棕色。通过紫外-可见分光光度法、傅里叶变换红外（FT-IR）和 X 射线衍射（XRD）分析证实了银纳米粒子的成功形成。紫外可见光谱和 XRD 的特征峰证实了 AgNPs 的合成。生物合成的 AgNPs 通过 DPPH 测定显示出潜在的抗氧化活性。这些 AgNPs 还对人体致病菌表现出潜在的抗菌活性。将结果与植物提取物的抗氧化和抗菌活性进行了比较，这清楚地表明，绿色生物合成的 AgNPs 可以构成有效的抗氧化和抗菌剂。
