Singh Reetika, Hano Christophe, Tavanti Francesco, Sharma Bechan
Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRA USC1328, Université d'Orléans, Eure et Loir Campus, 21 Rue de Loigny la Bataille, 28000 Chartres, France.
Materials (Basel). 2021 Nov 13;14(22):6854. doi: 10.3390/ma14226854.
Aubl. is an important medicinal tree. This tree is rich in various phytochemicals, and is therefore used as a potent antioxidant and antibacterial agent. This plant is also used for the treatment of various diseases. Here, we have improved its medicinal usage with the biosynthesis of silver nanoparticles (AgNPs) using Aubl. flower extract as a reducing and capping agent. The biosynthesis of the AgNPs reaction was carried out using 1 mM of silver nitrate and flower extract. The effect of the temperature on the biosynthesis of AgNPs was premeditated by room temperature (25 °C) and 60 °C. The continuous stirring of the reaction mixture at room temperature for approximately one hour resulted in the successful formation of AgNPs. A development of a yellowish brown color confirmed the formation of AgNPs. The efficacious development of AgNPs was confirmed by the characteristic peaks of UV-Vis, X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy spectra. The biosynthesized AgNPs exhibited significant free radical scavenging activity through a DPPH antioxidant assay. These AgNPs also showed potent antibacterial activity against many pathogenic bacterial species. The results of molecular dynamics simulations also proved the average size of NPs and antibacterial potential of the flower extract. The observations clearly recommended that the green biosynthesized AgNPs can serve as effective antioxidants and antibacterial agents over the plant extract.
奥布勒树是一种重要的药用树。这种树富含多种植物化学物质，因此被用作强效抗氧化剂和抗菌剂。这种植物还用于治疗各种疾病。在此，我们利用奥布勒树花提取物作为还原剂和封端剂通过生物合成银纳米颗粒（AgNPs）改进了其药用价值。使用1 mM硝酸银和花提取物进行AgNPs反应的生物合成。通过室温（25°C）和60°C预先考虑温度对AgNPs生物合成的影响。在室温下将反应混合物持续搅拌约一小时导致成功形成AgNPs。黄褐色的出现证实了AgNPs的形成。通过紫外可见光谱（UV-Vis）、X射线衍射（XRD）和傅里叶变换红外（FT-IR）光谱的特征峰证实了AgNPs的有效形成。通过DPPH抗氧化试验，生物合成的AgNPs表现出显著的自由基清除活性。这些AgNPs对许多病原菌也显示出强效抗菌活性。分子动力学模拟结果也证明了纳米颗粒的平均尺寸和花提取物的抗菌潜力。这些观察结果清楚地表明，绿色生物合成的AgNPs比植物提取物更能有效地作为抗氧化剂和抗菌剂。
