Wang Yusheng, Fang Zhifeng, Zhai Qixiao, Cui Shumao, Zhao Jianxin, Zhang Hao, Chen Wei, Lu Wenwei
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Microorganisms. 2021 Feb 22;9(2):452. doi: 10.3390/microorganisms9020452.
Vascular reactive oxygen species (ROS) play an essential role in cardiovascular diseases and the antioxidative effects of probiotics have been widely reported. To screen the probiotic strains that may prevent cardiovascular diseases, we tested the antioxidative effects of supernatants of different and strains on A7R5 cells. Preincubation with supernatants of CCFM752, CCFM1149, or CCFM10 significantly suppressed the angiotensin II-induced increases in ROS levels and increased catalase (CAT) activity in A7R5, whereas CCFM752 inhibited NADPH oxidase activation and CCFM1149 enhanced the intracellular superoxide dismutase (SOD) activity simultaneously. Treatment with CCFM752, CCFM1149, or CCFM10 supernatants had no significant impact on transcriptional levels of , , , , , or , but altered the overall transcriptomic profile and the expression of genes relevant to protein biosynthesis, and up-regulated the 60S ribosomal protein L7a (Rpl7a). A positive correlation between Rpl7a expression and intracellular CAT activity implied that Rpl7a may participate in CAT synthesis in A7R5. Supernatant of CCFM752 could also down-regulate the expression of NADPH oxidase activator 1 (Noxa1) and angiotensinogen in A7R5. Collectively, the probiotic strains CCFM752, CCFM1149, and CCFM10 exhibited antioxidative attributes on A7R5 cells and might help to reduce the risk of cardiovascular diseases.
血管活性氧（ROS）在心血管疾病中起重要作用，益生菌的抗氧化作用已被广泛报道。为筛选可能预防心血管疾病的益生菌菌株，我们测试了不同[具体菌株名称1]、[具体菌株名称2]和[具体菌株名称3]菌株的上清液对A7R5细胞的抗氧化作用。用[具体菌株名称1]CCFM752、[具体菌株名称2]CCFM1149或[具体菌株名称3]CCFM10的上清液预孵育可显著抑制血管紧张素II诱导的A7R5细胞中ROS水平升高，并增加过氧化氢酶（CAT）活性，而CCFM752抑制NADPH氧化酶激活，CCFM1149同时增强细胞内超氧化物歧化酶（SOD）活性。用CCFM752、CCFM1149或CCFM10的上清液处理对[相关基因名称1]、[相关基因名称2]、[相关基因名称3]、[相关基因名称4]、[相关基因名称5]或[相关基因名称6]的转录水平没有显著影响，但改变了整体转录组图谱以及与蛋白质生物合成相关的基因表达，并上调了60S核糖体蛋白L7a（Rpl7a）。Rpl7a表达与细胞内CAT活性之间的正相关表明Rpl7a可能参与A7R5细胞中CAT的合成。CCFM752的上清液还可下调A7R5细胞中NADPH氧化酶激活剂1（Noxa1）和血管紧张素原的表达。总的来说，益生菌菌株CCFM752、CCFM1149和CCFM10在A7R5细胞上表现出抗氧化特性，可能有助于降低心血管疾病风险。
