College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Plant Pathology of Hubei Province, Wuhan 430070, China.
Int J Mol Sci. 2022 May 28;23(11):6066. doi: 10.3390/ijms23116066.
spp. are important phytopathogenic fungi that infect a wide range of woody plants, resulting in big losses worldwide each year. However, their pathogenetic mechanisms and the related virulence factors are rarely addressed. In this study, seven lignin peroxidase (LiP) paralogs were detected in , named BkLiP1 to BkLiP7, respectively, while only BkLiP1 was identified as responsible for the vegetative growth and virulence of as assessed in combination with knock-out, complementation, and overexpression approaches. Moreover, BkLiP1, with the aid of a signal peptide (SP), is translocated onto the cell wall of and secreted into the apoplast space of plant cells as expressed in the leaves of , which can behave as a microbe-associated molecular pattern (MAMP) to trigger the defense response of plants, including cell death, reactive oxygen species (ROS) burst, callose deposition, and immunity-related genes up-regulated. It supports the conclusion that BkLiP1 plays an important role in the virulence and vegetative growth of and alternatively behaves as an MAMP to induce plant cell death used for the fungal version, which contributes to a better understanding of the pathogenetic mechanism of fungi.
种是重要的植物病原真菌，感染范围广泛的木本植物，每年在全球范围内造成巨大损失。然而，它们的发病机制和相关的毒力因子很少被涉及。在这项研究中，在 中检测到了七个木质素过氧化物酶（LiP）同工酶，分别命名为 BkLiP1 到 BkLiP7，而只有 BkLiP1 被确定为负责 的营养生长和毒力，这是通过敲除、互补和过表达方法评估的。此外，BkLiP1 在信号肽（SP）的帮助下，被转移到 的细胞壁上，并分泌到植物细胞的质外体空间中，如在 叶片中表达的那样，它可以作为一种微生物相关分子模式（MAMP）触发植物的防御反应，包括细胞死亡、活性氧（ROS）爆发、胼胝质沉积和免疫相关基因的上调。这支持了 BkLiP1 在 毒力和营养生长中发挥重要作用的结论，并且可以作为一种 MAMP 诱导植物细胞死亡，用于真菌版本，这有助于更好地理解 真菌的发病机制。
