Department of Epidemiology and Health Statistics, Fujian Medical University Fujian Provincial Key Laboratory of Environment Factors and Cancer, School of Public Health, Fujian Medical University, Fuzhou, 350122, China.
Department of Radiation Oncology, Anxi County Hospital, Quanzhou, 352400, China.
BMC Microbiol. 2021 Mar 6;21(1):73. doi: 10.1186/s12866-021-02137-x.
Microbiota has been reported to play a role in cancer patients. Nevertheless, little is known about the association between alcohol consumption and resultant changes in the diversity and composition of oesophageal microbiota in oesophageal squamous cell carcinoma (ESCC).
We performed a hospital-based retrospective study of 120 patients with pathologically diagnosed primary ESCC. The relevant information for all study participants were collected through a detailed questionnaire. The differences in adjacent tissues between non-drinkers and drinkers were explored using 16S rRNA gene sequencing. Raw sequencing data were imported into QIIME 2 to analyse the diversity and abundance of microbiota. Linear discriminant analysis effect size (LEfSe) and unconditional logistic regression were performed to determine the bacterial taxa that were associated with drinking.
The Shannon diversity index and Bray-Curtis distance of oesophageal microbiota were significantly different among drinkers(P < 0.05). The alcohol-related bacteria were primarily from the orders Clostridiales, Gemellales and Pasteurellales, family Clostridiaceae, Lanchnospiraceae, Helicobacteraceae, Alcaligenaceae, Bacteroidaceae, Pasteurellaceae and Gemellaceae; genus Clostridium, Helicobacter, Catonella, Bacteroides, Bacillus, Moraxella, and Bulleidia; and species B. moorei and longum (genus Bifidobacterium). In addition, the diversity and abundance of these microbiota were observed to be affected by the age, residential districts of the patients, and sampling seasons. Moreover, the higher the frequency and years of alcohol consumption, the lower was the relative abundance of genus Catonella that was observed.
Alcohol consumption is associated with alterations in both the diversity and composition the of the oesophageal microbiota in ESCC patients.
微生物群已被报道在癌症患者中发挥作用。然而，人们对饮酒与食管癌（ESCC）患者食管微生物多样性和组成变化之间的关系知之甚少。
我们对 120 名经病理诊断为原发性 ESCC 的患者进行了一项基于医院的回顾性研究。通过详细的问卷调查收集所有研究参与者的相关信息。使用 16S rRNA 基因测序探索非饮酒者和饮酒者之间的相邻组织差异。将原始测序数据导入 QIIME 2 以分析微生物多样性和丰度。采用线性判别分析效应量（LEfSe）和非条件逻辑回归确定与饮酒相关的细菌分类群。
饮酒者的食管微生物多样性的 Shannon 多样性指数和 Bray-Curtis 距离差异有统计学意义（P<0.05）。与酒精相关的细菌主要来自梭菌目、孪生菌目和巴斯德氏菌目、梭菌科、螺旋体科、螺旋杆菌科、阿克曼氏菌科、拟杆菌科、巴斯德氏菌科和孪生菌科；属梭菌属、螺旋杆菌属、卡托杆菌属、拟杆菌属、芽孢杆菌属、莫拉菌属和Bulleidia 属；种 B. moorei 和 longum（双歧杆菌属）。此外，这些微生物的多样性和丰度还受到患者年龄、居住地区和采样季节的影响。此外，饮酒频率和年限越高，观察到卡托杆菌属的相对丰度越低。
饮酒与 ESCC 患者食管微生物多样性和组成的改变有关。
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