Physical Chemistry and Applied Spectroscopy, Los Alamos National Laboratory, Los Alamos, NM, USA.
Applied Modern Physics, Los Alamos National Laboratory, Los Alamos, NM, USA.
Sci Rep. 2021 Mar 5;11(1):5287. doi: 10.1038/s41598-021-84353-z.
The separation of biomarkers from blood is straightforward in most molecular biology laboratories. However, separation in resource-limited settings, allowing for the successful removal of biomarkers for diagnostic applications, is not always possible. The situation is further complicated by the need to separate hydrophobic signatures such as lipids from blood. Herein, we present a microfluidic device capable of centrifugal separation of serum from blood at the point of need with a system that is compatible with biomarkers that are both hydrophilic and hydrophobic. The cross-flow filtration device separates serum from blood as efficiently as traditional methods and retains amphiphilic biomarkers in serum for detection.
从血液中分离生物标志物在大多数分子生物学实验室中都很简单。然而，在资源有限的环境中进行分离，以成功去除用于诊断应用的生物标志物，并不总是可行的。这种情况进一步复杂化了，因为需要从血液中分离出疏水性特征，如脂质。在此，我们提出了一种微流控装置，能够在需要的地方进行血清的离心分离，该系统与亲水性和疏水性生物标志物都兼容。该交叉流过滤装置能够像传统方法一样有效地将血清与血液分离，并保留血清中的两亲性生物标志物以进行检测。
